CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Tomer Ventura; Utpal Bose; Quinn P Fitzgibbon; Gregory G Smith; Nicholas Shaw; Scott F Cummins; Abigail Elizur

doi:10.1016/j.jsbmb.2017.04.007

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CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Journal article

Open access

Peer reviewed

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Tomer Ventura, Utpal Bose, Quinn P Fitzgibbon, Gregory G Smith, Nicholas Shaw, Scott F Cummins and Abigail Elizur

Journal of Steroid Biochemistry and Molecular Biology, Vol.171, pp.262-269

2017

DOI: https://doi.org/10.1016/j.jsbmb.2017.04.007

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Abstract

CYP450

ecdysone

juvenile hormone

decapoda

molt

metamorphosis

Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.

Details

Title: CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development
Authors: Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Utpal Bose (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Quinn P Fitzgibbon (Author) - University of Tasmania
Gregory G Smith (Author) - University of Tasmania
Nicholas Shaw (Author) - University of Queensland
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Journal of Steroid Biochemistry and Molecular Biology, Vol.171, pp.262-269
Publisher: Pergamon
Date published: 2017
DOI: 10.1016/j.jsbmb.2017.04.007
ISSN: 0960-0760
Grants: Redefining the molecular mechanism underlying crustacean metamorphosis, DP160103320, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451049702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Endocrinology & Metabolism

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