Journal article
CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development
Journal of Steroid Biochemistry and Molecular Biology, Vol.171, pp.262-269
2017
PMID: 28428023
Abstract
Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.
Details
- Title
- CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development
- Authors
- Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringUtpal Bose (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringQuinn P Fitzgibbon (Author) - University of TasmaniaGregory G Smith (Author) - University of TasmaniaNicholas Shaw (Author) - University of QueenslandScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Journal of Steroid Biochemistry and Molecular Biology, Vol.171, pp.262-269
- Publisher
- Pergamon
- Date published
- 2017
- DOI
- 10.1016/j.jsbmb.2017.04.007
- ISSN
- 0960-0760
- PMID
- 28428023
- Copyright note
- Copyright © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451049702621
- Output Type
- Journal article
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