CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences

I Katyal; Bonnie L Chaban; B Ng; J E Hill

doi:10.1007/s00248-013-0217-0

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CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences

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CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences

I Katyal, Bonnie L Chaban, B Ng and J E Hill

Microbial Ecology, Vol.66(1), pp.182-188

2013

DOI: https://doi.org/10.1007/s00248-013-0217-0

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Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) are currently a topic of interest in microbiology due to their role as a prokaryotic immune system. Investigations of CRISPR distribution and characterization to date have focused on pathogenic bacteria, while less is known about CRISPR in commensal bacteria, where they may have a significant role in the ecology of the microbiota of humans and other animals, and act as a recorder of interactions between bacteria and viruses. A combination of PCR and sequencing was used to determine prevalence and distribution of CRISPR arrays in Enterococcus faecalis and Enterococcus hirae isolates from the feces of healthy pigs. Both type II CRISPR-Cas and Orphan CRISPR (without Cas genes) were detected in the 195 isolates examined. CRISPR-Cas was detected in 52 (46/88) and 42 % (45/107) E. faecalis and E. hirae isolates, respectively. The prevalence of Orphan CRISPR arrays was higher in E. faecalis isolates (95 %, 84/88) compared with E. hirae isolates (49 %, 53/107). Species-specific repeat sequences were identified in Orphan CRISPR arrays, and 42 unique spacer sequences were identified. Only two spacers matched previously characterized pig virome sequences, and many were apparently derived from chromosomal sequences of enterococci. Surprisingly, 17 (40 %) of the spacers were detected in both species. Shared spacer sequences are evidence of a lack of species specificity in the agents and mechanisms responsible for integration of spacers, and the abundance of spacer sequences corresponding to bacterial chromosomal sequences reflects interspecific interactions within the intestinal microbiota. © 2013 Springer Science+Business Media New York.

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Title: CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences
Authors: I Katyal (Author) - University of Saskatchewan, Canada
Bonnie L Chaban (Author) - University of Saskatchewan, Canada
B Ng (Author) - University of Saskatchewan, Canada
J E Hill (Author) - University of Saskatchewan, Canada
Publication details: Microbial Ecology, Vol.66(1), pp.182-188
Publisher: Springer New York LLC
Date published: 2013
DOI: 10.1007/s00248-013-0217-0
ISSN: 0095-3628
Organisation Unit: University of the Sunshine Coast, Queensland; Thompson Institute; Centre for Bioinnovation
Language: English
Record Identifier: 99451009702621
Output Type: Journal article

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