CRISPR Diversity in E. coli Isolates from Australian Animals, Humans and Environmental Waters

Maxim Sheludchenko; Flavia Huygens; Helen Stratton; Megan Hargreave

doi:10.1371/journal.pone.0124090

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CRISPR Diversity in E. coli Isolates from Australian Animals, Humans and Environmental Waters

Journal article

Open access

Peer reviewed

CRISPR Diversity in E. coli Isolates from Australian Animals, Humans and Environmental Waters

Maxim Sheludchenko, Flavia Huygens, Helen Stratton and Megan Hargreave

PLoS One, Vol.10(5), e0124090

2015

DOI: https://doi.org/10.1371/journal.pone.0124090

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Abstract

e. coli genomes

Seventy four SNP genotypes and 54 E. coli genomes from kangaroo, Tasmanian devil, reptile, cattle, dog, horse, duck, bird, fish, rodent, human and environmental water sources were screened for the presence of the CRISPR 2.1 loci flanked by cas2 and iap genes. CRISPR 2.1 regions were found in 49% of the strains analysed. The majority of human E. coli isolates lacked the CRISPR 2.1 locus. We described 76 CRISPR 2.1 positive isolates originating from Australian animals and humans, which contained a total of 764 spacer sequences. CRISPR arrays demonstrated a long history of phage attacks especially in isolates from birds (up to 40 spacers). The most prevalent spacer (1.6%) was an ancient spacer found mainly in human, horse, duck, rodent, reptile and environmental water sources. The sequence of this spacer matched the intestinal P7 phage and the pO111 plasmid of E. coli.

Details

Title: CRISPR Diversity in E. coli Isolates from Australian Animals, Humans and Environmental Waters
Authors: Maxim Sheludchenko (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Flavia Huygens (Author) - Queensland University of Technology
Helen Stratton (Author) - Griffith University
Megan Hargreave (Author) - Queensland University of Technology
Publication details: PLoS One, Vol.10(5), e0124090; 12
Publisher: Public Library of Science
Date published: 2015
DOI: 10.1371/journal.pone.0124090
ISSN: 1932-6203
Copyright note: Copyright © 2015 Sheludchenko et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449130002621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Microbiology

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