C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis

Haichen Long; Yangyang Li; Huijuan Wang; Bingxuan Guo; Shuyan Song; Xiangyi Zhe; Hongtao Li; Dongmei Li; Renfu Shao; Zemin Pan

doi:10.1186/s12885-023-10543-9

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C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis

Journal article

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Peer reviewed

C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis

Haichen Long, Yangyang Li, Huijuan Wang, Bingxuan Guo, Shuyan Song, Xiangyi Zhe, Hongtao Li, Dongmei Li, Renfu Shao and Zemin Pan

BMC Cancer, Vol.23(1), pp.1-13

2023

DOI: https://doi.org/10.1186/s12885-023-10543-9

PMCID: PMC9872280

PMID: 36694148

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Abstract

Cervical cancer

Gene expression

Invasion

Migration

Proliferation

C/EBPβ

Background: Cervical cancer is currently estimated to be the fourth most common cancer among women worldwide and the leading cause of cancer-related deaths in some of the world’s poorest countries. C/EBPβ has tumor suppressor efects because it is necessary for oncogene induced senescence. However, C/EBPβ also has an oncogenic role. The specifc role of C/EBPβ in cervical cancer as a tumor suppressor or oncoprotein is unclear. Objective: To explore the role of the C/EBPβ protein in cervical tumorigenesis and progression. Methods: Quantitative RT PCR was used to analyze C/EBPβ (15 cervical cancer tissue samples and 15 corresponding normal cervical tissue samples), miR 661, and MTA1 mRNA expression in clinical samples (10 cervical cancer tissue samples and 10 corresponding normal cervical tissue samples). Immunohistochemistry was used to analyze C/ EBPβ (381 clinical samples), Ki67 (80 clinical samples) and PCNA ( 60 clinical samples) protein expression. MALDI TOF MassARRAY was used to analyze C/EBPβ gene methylation (13 cervical cancer tissues and 13 corresponding normal cervical tissues). Cell proliferation was analyzed by CCK 8 in cervical cancer cell lines. Western blotting and immunohistochemistry were performed to detect C/EBPβ protein expression levels, and mRNA expression was analyzed by quantitative RT PCR analysis. Flow cytometry was performed to measure cell cycle distribution and cell apoptosis. Colony formation, Transwell, cell invasion, and wound healing assays were performed to detect cell migration and invasion. Results: C/EBPβ protein expression was signifcantly reduced in cervical cancer tissues compared with cervicitis tissues (P<0.01). Ki67 protein and PCNA protein expression levels were signifcantly higher in cervical cancer tissues compared with cervicitis tissues. The rate of C/EBPβ gene promoter methylation of CpG12, 13, 14 and CpG19 in cervical cancer tissues was signifcantly increased compared with normal cervical tissue (P<0.05). In addition, C/EBPβ was overexpressed in cervical cancer cells and this overexpression inhibited cell proliferation, migration, invasion, arrested cells in S phase, and promoted apoptosis. Conclusions: We have demonstrated that C/EBPβ decreased in cervical cancer tissues and overexpression of the C/EBPβ gene in cervical cancer cells could inhibit proliferation, invasion and migration.

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Title: C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis
Authors: Haichen Long (Author) - Shihezi University
Yangyang Li (Author) - Shihezi University
Huijuan Wang (Author) - Shihezi University
Bingxuan Guo (Author) - Shihezi University
Shuyan Song (Author) - Shihezi University
Xiangyi Zhe (Corresponding Author) - Shihezi University
Hongtao Li (Author) - Shihezi University
Dongmei Li (Author) - Shihezi University
Renfu Shao (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Zemin Pan (Corresponding Author) - Shihezi University
Publication details: BMC Cancer, Vol.23(1), pp.1-13
Publisher: BioMed Central Ltd.
Date published: 2023
DOI: 10.1186/s12885-023-10543-9
ISSN: 1471-2407
PMID: 36694148; PMC9872280
Copyright note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Grant note: 82060518, U1503125/ National Natural Science Foundation of China 2019BC007/ International Science and Technology
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99711298802621
Output Type: Journal article

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