Journal article
Burkholderia pseudomallei lipopolysaccharide genotype does not correlate with severity or outcome in melioidosis: host risk factors remain the critical determinant
Open Forum Infectious Diseases, Vol.6(4), ofz091
2019
Abstract
Background: The causative agent of melioidosis is the Gram-negative bacterium Burkholderia pseudomallei. Clinical presentations of melioidosis are notably diverse, with host risk factors considered central to progression from infection to disease and clinical outcome. Ubiquitous and variably present virulence determinants have been described for B. pseudomallei, with several variably present minority genotypes associated with specific disease presentations. The lipopolysaccharide (LPS) O-antigen of B. pseudomallei is highly diverse with four types described. Invitro data suggest differential virulence between LPS types but it remains unclear whether this LPS O-antigen diversity influences clinical presentation, severity and outcomes in patients with melioidosis. Methods: Whole-genome sequencing was performed to assign an LPS type to 1005 consecutive B. pseudomallei strains, each corresponding to a melioidosis patient enrolled in the 28 year Darwin Prospective Melioidosis study. Correlations of LPS genotype with clinical parameters was then undertaken. Results: Bivariate analysis demonstrated that mortality and the rates of bacteremia and septic shock were the same for patients with the two predominant B. pseudomallei LPS genotypes A (87% of cases) and B (12% of all cases). Mortality was 12% and 12%, bacteremia 57% and 53% and septic shock 22% and 18% for LPS A and LPS B, respectively. Conclusions: LPS genotype was not associated with melioidosis severity or outcome. These findings suggest that in vitro differential virulence between B. pseudomallei LPS genotypes does not translate to clinical significance and this supports the primary role of host risk factors in determining disease severity and outcomes in melioidosis.
Details
- Title
- Burkholderia pseudomallei lipopolysaccharide genotype does not correlate with severity or outcome in melioidosis: host risk factors remain the critical determinant
- Authors
- Jessica R Webb (Corresponding Author) - Menzies School of Health ResearchDerek S Sarovich (Author) - University of the Sunshine CoastErin P Price (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyLinda M Ward (Author) - Menzies School of Health ResearchMark Mayo (Author) - Menzies School of Health ResearchBart J Currie (Author) - Menzies School of Health Research
- Publication details
- Open Forum Infectious Diseases, Vol.6(4), ofz091
- Publisher
- Oxford University Press
- Date published
- 2019
- DOI
- 10.1093/ofid/ofz091
- ISSN
- 2328-8957
- Copyright note
- Copyright © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450779502621
- Output Type
- Journal article
Metrics
39 File views/ downloads
461 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Immunology
- Infectious Diseases
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites