Journal article
Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides
Antimicrobial Agents and Chemotherapy, Vol.58(1), pp.162-166
2014
Abstract
Melioidosis is a potentially fatal disease caused by the saprophytic bacterium Burkholderia pseudomallei. Resistance to gentamicin is generally a hallmark of B. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. In this study, we determined the prevalence and mechanism of gentamicin susceptibility found in B. pseudomallei isolates from Sarawak, Malaysian Borneo. We performed multilocus sequence typing and antibiotic susceptibility testing on 44 B. pseudomallei clinical isolates from melioidosis patients in Sarawak district hospitals. Whole-genome sequencing was used to identify the mechanism of gentamicin susceptibility. A novel allelic-specific PCR was designed to differentiate gentamicin-sensitive isolates from wild-type B. pseudomallei. A reversion assay was performed to confirm the involvement of this mechanism in gentamicin susceptibility. A substantial proportion (86%) of B. pseudomallei clinical isolates in Sarawak, Malaysian Borneo, were found to be susceptible to the aminoglycoside gentamicin, a rare occurrence in other regions where B. pseudomallei is endemic. Gentamicin sensitivity was restricted to genetically related strains belonging to sequence type 881 or its single-locus variant, sequence type 997. Whole-genome sequencing identified a novel nonsynonymous mutation within amrB, encoding an essential component of the AmrAB-OprA multidrug efflux pump. We confirmed the role of this mutation in conferring aminoglycoside and macrolide sensitivity by reversion of this mutation to the wild-type sequence. Our study demonstrates that alternative B. pseudomallei selective media without gentamicin are needed for accurate melioidosis laboratory diagnosis in Sarawak. This finding may also have implications for environmental sampling of other locations to test for B. pseudomallei endemicity. © 2014, American Society for Microbiology. All Rights Reserved.
Details
- Title
- Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides
- Authors
- Y Podin (Author) - Menzies School of Health ResearchDerek S Sarovich (Author) - Menzies School of Health ResearchErin P Price (Author) - Menzies School of Health ResearchM Kaestli (Author) - Menzies School of Health ResearchM Mayo (Author) - Menzies School of Health ResearchK Hii (Author) - Kapit Hospital, MalaysiaN HieUng (Author) - Sibu Hospital, MalaysiaS Wong (Author) - Sibu Hospital, MalaysiaI Wong (Author) - Sibu Hospital, MalaysiaJ Wong (Author) - Bintulu Hospital, MalaysiaA Mohan (Author) - Bintulu Hospital, MalaysiaM Ooi (Author) - University Malaysia Sarwak, MalaysiaT Fam (Author) - Miri Hospital, MalaysiaJ Wong (Author) - Miri Hospital, MalaysiaA Tuanyok (Author) - Northern Arizona University, United StatesP Keim (Author) - Menzies School of Health ResearchP M Giffard (Author) - Northern Arizona University, United StatesB J Currie (Author) - Menzies School of Health Research
- Publication details
- Antimicrobial Agents and Chemotherapy, Vol.58(1), pp.162-166
- Publisher
- American Society for Microbiology
- Date published
- 2014
- DOI
- 10.1128/AAC.01842-13
- ISSN
- 0066-4804
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450520702621
- Output Type
- Journal article
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