Burkholderia paludis sp. nov., an antibiotic-siderophore producing novel Burkholderia cepacia complex species, isolated from malaysian tropical peat swamp soil

K S Ong; Y K Aw; L H Lee; Catherine M Yule; Y L Cheow; S M Lee

doi:10.3389/fmicb.2016.02046

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Burkholderia paludis sp. nov., an antibiotic-siderophore producing novel Burkholderia cepacia complex species, isolated from malaysian tropical peat swamp soil

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Burkholderia paludis sp. nov., an antibiotic-siderophore producing novel Burkholderia cepacia complex species, isolated from malaysian tropical peat swamp soil

K S Ong, Y K Aw, L H Lee, Catherine M Yule, Y L Cheow and S M Lee

Frontiers in Microbiology, Vol.7, 2046

2016

DOI: https://doi.org/10.3389/fmicb.2016.02046

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Abstract

antimicrobial

burkholderia cepacia complex

multilocus sequence analysis (MLSA)

polyphasic taxonomy

tropical peat swamp forest

A novel Gram negative rod-shaped bacterium, designated strain MSh1T, was isolated from Southeast Pahang tropical peat swamp forest soil in Malaysia and characterized using a polyphasic taxonomy approach. The predominant cellular fatty acids (> 10.0%) were C16:0 (31.7%), C17:0 cyclo (26.6%), and C19:0 cyclo ω8c (16.1%). The polar lipids detected were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The predominant ubiquinone was Q-8. This revealed that strain MSh1T belongs to the genus Burkholderia. The type strain MSh1T can be differentiated from other Burkholderia cepacia complex (Bcc) species by phylogenetic analysis of 16S rRNA gene sequence, multilocus sequence analysis (MLSA), average nucleotide identity (ANI) and biochemical tests. DNA-DNA relatedness values between strain MSh1T and closely related type strains were below the 70% threshold value. Based on this polyphasic study of MSh1T, it can be concluded that this strain represents a novel species within the Bcc, for which the name Burkholderia paludis sp. nov. is proposed. The type strain is MSh1T (= DSM 100703T = MCCC 1K01245T). The dichloromethane extract of MSh1T exhibited antimicrobial activity against four Gram positive bacteria (Enterococcus faecalis ATCC 29212, E. faecalis ATCC 700802, Staphylococcus aureus ATCC 29213, S. aureus ATCC 700699) and a Gram negative bacteria (Escherichia coli ATCC 25922). Further purification work has led to the isolation of Compound 1, pyochelin. Pyochelin demonstrated antimicrobial activity against four S. aureus strains and three E. faecalis strains with MIC-values of 3.13 μg/ml and 6.26 μg/ml, respectively. SEM analysis showed that the cellular morphology of E. faecalis ATCC 700802 was not affected by pyochelin; suggesting that it might target the intracellular components. Pyochelin, a siderophore with antimicrobial activity might be useful in treating bacterial infections caused by S. aureus and E. faecalis, however further work has to be done. © 2016 Ong, Aw, Lee, Yule, Cheow and Lee.

Details

Title: Burkholderia paludis sp. nov., an antibiotic-siderophore producing novel Burkholderia cepacia complex species, isolated from malaysian tropical peat swamp soil
Authors: K S Ong (Author) - Monash University Malaysia, Malaysia
Y K Aw (Author) - Monash University Malaysia, Malaysia
L H Lee (Author) - Monash University Malaysia, Malaysia
Catherine M Yule (Author) - Monash University Malaysia, Malaysia
Y L Cheow (Author) - Monash University Malaysia, Malaysia
S M Lee (Author) - Monash University Malaysia, Malaysia
Publication details: Frontiers in Microbiology, Vol.7, 2046; 14
Publisher: Frontiers Research Foundation
Date published: 2016
DOI: 10.3389/fmicb.2016.02046
ISSN: 1664-302X
Copyright note: Copyright © 2016 Ong, Aw, Lee, Yule, Cheow and Lee. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99450444002621
Output Type: Journal article

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