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Epilepsia Open - 2022 - Seo - Brain glycogen content is increased in the acute and interictal chronic stages of the mouse1.35 MB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Brain glycogen content is increased in the acute and interictal chronic stages of the mouse pilocarpine model of epilepsy
Epilepsia Open, Vol.7(2), pp.361-367
2022
PMID: 35377551
Abstract
Glucose is the main brain fuel in fed conditions, while astrocytic glycogen is used as supplemental fuel when the brain is stimulated. Brain glycogen levels are decreased shortly after induced seizures in rodents, but little is known about how glycogen levels are affected interictally in chronic models of epilepsy. Reduced glutamine synthetase activity has been suggested to lead to increased brain glycogen levels in humans with chronic epilepsy. Here, we used the mouse pilocarpine model of epilepsy to investigate whether brain glycogen levels are altered, both acutely and in the chronic stage of the model. One day after pilocarpine-induced convulsive status epilepticus (CSE), glycogen levels were higher in the hippocampal formation, cerebral cortex, and cerebellum. Opposite to expected, this was accompanied by elevated glutamine synthetase activity in the hippocampus but not the cortex. Increased interictal glycogen amounts were seen in the hippocampal formation and cerebral cortex in the chronic stage of the model (21 days post-CSE), suggesting long-lasting alterations in glycogen metabolism. Glycogen solubility in the cerebral cortex was unaltered in this epilepsy mouse model. Glycogen synthase kinase 3 beta (Gsk3b) mRNA levels were reduced in the hippocampal formations of mice in the chronic stage, which may underlie the elevated brain glycogen content in this model. This is the first report of elevated interictal glycogen levels in a chronic epilepsy model. Increased glycogen amounts in the brain may influence seizure susceptibility in this model, and this warrants further investigation.
Details
- Title
- Brain glycogen content is increased in the acute and interictal chronic stages of the mouse pilocarpine model of epilepsy
- Authors
- Gi Young Seo - The University of QueenslandElliott S Neal - The University of QueenslandFelicity Han - The University of QueenslandDiana Vidovic - The University of QueenslandFathima Nooru-Mohamed - The University of QueenslandGerald A Dienel - University of Arkansas for Medical SciencesMitchell A Sullivan - The University of QueenslandKarin Borges (Corresponding Author) - The University of Queensland
- Publication details
- Epilepsia Open, Vol.7(2), pp.361-367
- Publisher
- Wiley-Blackwell Publishing, Inc.
- Date published
- 2022
- DOI
- 10.1002/epi4.12599
- ISSN
- 2470-9239
- PMID
- 35377551
- Copyright note
- This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2022 The Authors. Epilepsia Open published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035093602621
- Output Type
- Journal article
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- Web Of Science research areas
- Clinical Neurology
- Neurosciences
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