Journal article
Biomolecular changes that occur in the antennal gland of the giant freshwater prawn (Machrobrachium rosenbergii)
PLoS One, Vol.12(6), pp.1-23
2017
PMCID: PMC5490968
PMID: 28662025
Abstract
In decapod crustaceans, the antennal gland (AnG) is a major primary source of externally secreted biomolecules, and some may act as pheromones that play a major role in aquatic animal communication. In aquatic crustaceans, sex pheromones regulate reproductive behaviours, yet they remain largely unidentified besides the N-acetylglucosamine-1,5-lactone (NAGL) that stimulates male to female attraction. In this study, we used an AnG transcriptome of the female giant freshwater prawn (Macrobrachium rosenbergii) to predict the secretion of 226 proteins, including the most abundantly expressed transcripts encoding the Spaetzle protein, a serine protease inhibitor, and an arthropodial cuticle protein AMP 8.1. A quantitative proteome analysis of the female AnG at intermolt, premolt and postmolt, identified numerous proteins of different abundances, such as the hemocyanin subunit 1 that is most abundant at intermolt. We also show that hemocyanin subunit 1 is present within water surrounding females. Of those metabolites identified, we demonstrate that the NAGL and Nacetylglucosamine (NAG) can bind with high affinity to hemocyanin subunit 1. In summary, this study has revealed components of the female giant freshwater prawn AnG that are released and contribute to further research towards understanding crustacean conspecific signalling.
Details
- Title
- Biomolecular changes that occur in the antennal gland of the giant freshwater prawn (Machrobrachium rosenbergii)
- Authors
- Utpal Bose (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringThanapong Kruangkum (Author) - Mahidol UniversityTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMin Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringShahida Akter Mitu (Author) - University of the Sunshine Coast, QueenslandMark P Hodson (Author) - University of QueenslandPaul N Shaw (Author) - The University of QueenslandPrasert Sobhon (Author) - Mahidol UniversityScott F Cummins (Author) - University of the Sunshine Coast, Queensland
- Publication details
- PLoS One, Vol.12(6), pp.1-23
- Publisher
- Public Library of Science
- Date published
- 2017
- DOI
- 10.1371/journal.pone.0177064
- ISSN
- 1932-6203
- PMID
- 28662025; PMC5490968
- Copyright note
- Copyright © 2017 Bose et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450560402621
- Output Type
- Journal article
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