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Between-day reliability of cytokines and adipokines for application in research and practice832.23 kB
Published VersionCC BY V4.0, Open Access
Journal article
Between-day reliability of cytokines and adipokines for application in research and practice
Frontiers in Physiology, Vol.13, pp.1-11
2022
Abstract
Purpose: This study assessed the biological reliability of peripheral human cytokines and adipokines, and the influence of participant characteristics on total error. This has essential application to interventional cytokine measurement to ensure that reported results are interpreted with confidence.Methods: Participants (49% female, 18–85 years, n = 84) completed two consecutive-day testing sessions. Participants provided a venous blood sample at the same time of day across two consecutive days, under standardized participant presentation, including 24-h rested and 12-h fasted conditions. Multiplex immunoassay was used to assess inflammatory analytes from samples (predominantly plasma). Repeat measurements were conducted between-day for total precision quantification, and technical (technique) error was negated from the total to provide an estimate of biological (attributed to participant presentation) error.Results: Whilst there was no evidence of statistically significant biological error, a small amount of biological error was consistently present across most analytes (∼3.3%/0.07 pg/ml), which was largest for measurement of leptin (7.3%/210 pg/ml). There was also an influence of sex on reliability of leptin and adiponectin (total model explained 6–7% of error variation), where females demonstrated the greatest error.Conclusion: Biological error reported in this study should be applied to any future study or individual with a repeated measurement of cytokine concentrations over time that maintain best practice procedures (12-h fasted, 24-h rested). In most cases, raw error should be used, with exceptions for women for measurement of leptin and adiponectin. This approach will ensure that results are reported with certainty for improved reporting of intervention efficacy.
Details
- Title
- Between-day reliability of cytokines and adipokines for application in research and practice
- Authors
- Grace L Rose (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacyMorgan J Farley (Author) - The University of QueenslandNicole B. Flemming (Author) - School of Medicine and Dentistry, Griffith University, Birtinya, QLD, AustraliaTina L Skinner (Author) - The University of QueenslandMia Schaumberg (Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - Legacy
- Publication details
- Frontiers in Physiology, Vol.13, pp.1-11
- Publisher
- Frontiers Research Foundation
- Date published
- 2022
- DOI
- 10.3389/fphys.2022.967169
- ISSN
- 1664-042X
- Copyright note
- © 2022 Rose, Farley, Flemming, Skinner and Schaumberg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Health - Biomedicine; School of Health; University of the Sunshine Coast, Queensland; Cancer Research Cluster; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99667898902621
- Output Type
- Journal article
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