Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities

Renata L Grunennvaldt; J Degenhardt-Goldbach; J de C Tomasi; G Davila Dos Santos; V Aparecida Vicente; C Deschamps

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Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities

Journal article

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Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities

Renata L Grunennvaldt, J Degenhardt-Goldbach, J de C Tomasi, G Davila Dos Santos, V Aparecida Vicente and C Deschamps

Biotecnología Vegetal, Vol.18(1), pp.3-13

2018

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Abstract

Horticultural Production

yerba mate

16S rRNA

sequencing

tissue culture

Endophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests.

Details

Title: Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities
Authors: Renata L Grunennvaldt (Author) - Federal University of Parana, Brazil
J Degenhardt-Goldbach (Author) - National Centre of Forestry Research, Brazil
J de C Tomasi (Author) - Federal University of Parana, Brazil
G Davila Dos Santos (Author) - Federal University of Parana, Brazil
V Aparecida Vicente (Author) - Federal University of Parana, Brazil
C Deschamps (Author) - Federal University of Parana, Brazil
Publication details: Biotecnología Vegetal, Vol.18(1), pp.3-13
Publisher: Instituto de Biotecnologia de las Plantas
Date published: 2018
ISSN: 2074-8647
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99451274002621
Output Type: Journal article

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