Babesia bovis: Molecular and biological characteristics of cloned parasite lines

A C Gill; A F Cowman; N P Stewart; D J Kemp; Peter Timms

doi:10.1016/0014-4894(87)90160-3

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Babesia bovis: Molecular and biological characteristics of cloned parasite lines

Journal article

Peer reviewed

Babesia bovis: Molecular and biological characteristics of cloned parasite lines

A C Gill, A F Cowman, N P Stewart, D J Kemp and Peter Timms

Experimental Parasitology, Vol.63(2), pp.180-188

1987

DOI: https://doi.org/10.1016/0014-4894(87)90160-3

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Abstract

babesia bovis

protozoa

parasitic

babesiosis

bovine

An in vivo limiting dilution technique was used to produce several Babesia bovis cloned lines with which to study the basis of virulence and immunogenicity in this parasite. DNA hybridization using a cloned DNA fragment from the BabR locus demonstrated that the cloned lines were a more restricted genetic population than the parent strain. Biosynthetic labeling and immunoprecipitation studies indicated that the cloned lines differed from each other and from the parentals in the expression of a small number of polypeptides and antigens. Animal trials with three of the lines demonstrated that the parental line contains both virulent and avirulent parasite populations, at least three of which are not tick transmissible, and that while the lines do provide significant protection against heterologous challenge, they may not give as effective protection as the parental line. These experiments demonstrated the existence of subpopulations with distinctive molecular and biological properties, providing evidence that the attenuation process is based on the selection of preexisting parasite subpopulations combined with the ability of these parasites to vary genetically. © 1987.

Details

Title: Babesia bovis: Molecular and biological characteristics of cloned parasite lines
Authors: A C Gill (Author) - Department of Primary Industries
A F Cowman (Author) - Department of Primary Industries
N P Stewart (Author) - Department of Primary Industries
D J Kemp (Author) - Department of Primary Industries
Peter Timms (Author) - Department of Primary Industries
Publication details: Experimental Parasitology, Vol.63(2), pp.180-188
Publisher: Academic Press
Date published: 1987
DOI: 10.1016/0014-4894(87)90160-3
ISSN: 0014-4894
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449992302621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Parasitology

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