Journal article
BCoR-L1 variation and breast cancer
Breast Cancer Research, Vol.9(4), R54
2007
Abstract
Introduction: BRCA1 is involved in numerous essential processes in the cell, and the effects of BRCA1 dysfunction in breast cancer carcinogenesis are well described. Many of the breast cancer susceptibility genes such as BRCA2, p53, ATM, CHEK2, and BRIP1 encode proteins that interact with BRCA1. BCL6 corepressor-like 1 (BCoR-L1) is a newly described BRCA1-interacting protein that displays high homology to several proteins known to be involved in the fundamental processes of DNA damage repair and transcription regulation. BCoR-L1 has been shown to play a role in transcription corepression, and expression of the X-linked BCoR-L1 gene has been reported to be dysregulated in breast cancer subjects. BCoR-L1 is located on the X chromosome and is subject to X inactivation. Methods: We performed mutation analysis of 38 BRCA1/2 mutation-negative breast cancer families with male breast cancer, prostate cancer, and/or haplotype sharing around BCoR-L1 to determine whether there is a role for BCoR-L1 as a high-risk breast cancer predisposition gene. In addition, we conducted quantitative real-time PCR (qRT-PCR) on lymphoblastoid cell lines (LCLs) from the index cases from these families and a number of cancer cell lines to assess the role of BCoR-L1 dysregulation in cancer and cancer families. Results: Very little variation was detected in the coding region, and qRT-PCR analysis revealed that BCoR-L1 expression is highly variable in cancer-free subjects, high-risk breast cancer patients, and cancer cell lines. We also report the investigation of a new expression control, DIDO1 (death inducer-obliterator 1), that is superior to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and UBC (ubiquitin C) for analysis of expression in LCLs. Conclusion: Our results suggest that BCoR-L1 expression does not play a large role in predisposition to familial breast cancer. © 2007 Lose et al.; licensee BioMed Central Ltd.
Details
- Title
- BCoR-L1 variation and breast cancer
- Authors
- Felicity Lose (Author) - University of QueenslandJ Arnold (Author) - Queensland Institute of Medical ResearchD B Young (Author) - Queensland Institute of Medical ResearchC J Brown (Author) - University of British Columbia, CanadaG J Mann (Author) - University of SydneyG M Pupo (Author) - University of SydneyK K Khanna (Author) - Queensland Institute of Medical ResearchG Chenevix-Trench (Author) - Queensland Institute of Medical ResearchA B Spurdle (Author) - Queensland Institute of Medical Research
- Publication details
- Breast Cancer Research, Vol.9(4), R54
- Publisher
- BioMed Central Ltd.
- Date published
- 2007
- DOI
- 10.1186/bcr1759
- ISSN
- 1465-5411
- Copyright note
- Copyright © 2007 Lose et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of Research
- Language
- English
- Record Identifier
- 99450701602621
- Output Type
- Journal article
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