Azure a mediated polypyrrole-based amperometric nitrate biosensor

S B Adeloju; Manzar Sohail

doi:10.1002/elan.201000386

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Azure a mediated polypyrrole-based amperometric nitrate biosensor

Journal article

Peer reviewed

Azure a mediated polypyrrole-based amperometric nitrate biosensor

S B Adeloju and Manzar Sohail

Electroanalysis, Vol.23(4), pp.987-996

2011

DOI: https://doi.org/10.1002/elan.201000386

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Abstract

nitrate biosensor

nitrate reductase

Azure A

amperometry

polypyrrole

The co-entrapment of Aspergillus niger-nitrate reductase (NaR) and azure A (AzA), as a redox mediator has been investigated for fabrication of a polypyrrole (PPy)-based electron mediated amperometric nitrate biosensor. Optimum formation of PPy-NaR-AzA film was achieved in a monomer solution which contained 0.3M Py, 0.1M KCl, 1U/mL NaR, 50μM AzA with an applied current density of 0.5mA/cm 2 for 240s. The optimum applied potential of -0.25V for amperometric detection of nitrate with PPy-NaR-AzA biosensor was 50, 100 and 150mV more positive than those achieved with the use of safranin O (Saf), methyl viologen (MV) and bromophenol blue (BrB) or red (BrR), respectively, as mediators. Similar optimum applied of -0.25V was achieved with thionin acetate (ThAc) which was in the same phenothiazine group of mediators as AzA. The electron mediated amperometric detection of nitrate with the PPy-NaR-AzA biosensor also gave the best performance with a sensitivity of 40.8nA μM -1 cm -2, detection limit of 0.5μM, a response time of 18-20s and a linear concentration range of 20-500μM.

Details

Title: Azure a mediated polypyrrole-based amperometric nitrate biosensor
Authors: S B Adeloju (Author) - Monash University
Manzar Sohail (Author) - Monash University
Publication details: Electroanalysis, Vol.23(4), pp.987-996
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Date published: 2011
DOI: 10.1002/elan.201000386
ISSN: 1040-0397
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99448951702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Chemistry, Analytical; Electrochemistry

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