Journal article
Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments
Marine Biotechnology, Vol.1(2), pp.175-183
1999
Abstract
The association of foreign DNA with gilthead seabream (Sparus aurata) sperm was enhanced relative to simple coincubation by sonication, freezing, dimethyl sulfoxide and polyethylene glycol treatments. Sonication yielded the strongest dot blot signals, equivalent to 250 to 380 foreign DNA copies per spermatozoa. We are unaware of previous reports attempting to associate DNA with ultrasound for fish or elsewhere. However, no or negligible foreign DNA was evident in 1- and 2-day-old fish larvae resulting from eggs fertilized with sonicated or frozen sperm.
Details
- Title
- Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments
- Authors
- X Y Liu (Author) - National Centre for MaricultureY Zohar (Author) - National Centre for MaricultureWayne R Knibb (Author) - National Centre for Mariculture
- Publication details
- Marine Biotechnology, Vol.1(2), pp.175-183
- Publisher
- Springer New York LLC
- Date published
- 1999
- DOI
- 10.1007/PL00011765
- ISSN
- 1436-2228
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449907902621
- Output Type
- Journal article
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- Marine & Freshwater Biology
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