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Journal article
Assessing Molecular Mechanisms of Stress Induced Salinity Adaptation in the Juvenile Ornate Spiny Lobster, Panulirus ornatus
International Journal of Molecular Sciences, Vol.26(22), pp.1-28
2025
Abstract
Panulirus ornatus, the ornate spiny lobster, is a stenohaline weak hyper-osmoregulator, yet its osmoregulatory response to salinity stress remains poorly understood. This study investigated six osmoregulatory genes—Na+/K+-ATPase (nka), V-type H+-ATPase (vhe), Na+/HCO3− exchanger (nbc), Na+/K+/2Cl− co-transporter (nkcc), Na+/H+ exchanger (nhe), and carbonic anhydrase (ca)—in juvenile gills exposed to 25 ppt, 34 ppt (control), and 40 ppt salinities during acute (48 h) and chronic (>38 d) phases. Transcriptome analysis revealed that all genes were unresponsive following either 25 ppt or 40 ppt salinity acute exposure. However, nkcc showed a tendency toward for upregulation under 25 ppt salinity during acute exposure. Additionally, glutathione S-transferase and putative ferrous reductase 1 were upregulated under 25 ppt salinity, suggesting increased metabolic demand. In contrast, glutathione peroxidase and an ammonia transporter were upregulated in 40 ppt salinity, indicating protein catabolism. Quantitative PCR confirmed nkcc- and nka upregulation under chronic 25 ppt salinity. Vhe, nbc, nhe and ca showed no response, and 40 ppt salinity did not affect the six target genes. These findings suggest P. ornatus relies on nkcc- and nka-mediated ion transport and lacks mechanisms to tolerate high salinity, resulting in reduced growth and survival. These findings define optimal salinity range for aquaculture (25–34 ppt), highlighting the need to avoid high-salinity stress in lobster water quality management
Details
- Title
- Assessing Molecular Mechanisms of Stress Induced Salinity Adaptation in the Juvenile Ornate Spiny Lobster, Panulirus ornatus
- Authors
- Eleanor L. Spencer - University of TasmaniaQuinn P. Fitzgibbon - University of TasmaniaSusan Glendinning - University of the Sunshine Coast, Queensland, Centre for BioinnovationCourtney L. Lewis - University of the Sunshine Coast, Queensland, Centre for BioinnovationThomas M. Banks - University of TasmaniaAndrew J. Trotter - University of TasmaniaTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationGregory G. Smith - University of Tasmania
- Publication details
- International Journal of Molecular Sciences, Vol.26(22), pp.1-28
- Publisher
- MDPI AG
- Date published
- 2025
- DOI
- 10.3390/ijms262211150
- ISSN
- 1422-0067; 1661-6596
- Copyright note
- © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- The transcriptomic data presented in this study can be found on CrustyBase.org, accessed on 17 December 2023. The data has also been uploaded to the NCBI sequence read archive under the following BioProject number: PRJNA1356957. Transcripts DEG annotations and phylogenetic tree accession numbers have been made available in the Supplementary Material (Tables S1 and S2). Physiological and qPCR data will be made available on request.
- Organisation Unit
- School of Education and Tertiary Access; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991184303102621
- Output Type
- Journal article
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