Journal article
Assay and properties of 1,3-Beta-glucanase in soil
Soil Biology and Biochemistry, Vol.10(5), pp.389-391
1978
Abstract
1.3-Beta-Glucanase (laminarinase) activity in soil was measured using laminarin as the substrate. Activity was optimal in sodium acid-maleate buffer at pH 5.4 and followed Michaelis-Menten kinetics. Three methods of analysing kinetic data gave Km values of 0.23, 0.21 and 0.20 mg.ml-1. Vmax values were 0.41, 0.39 and 0.39 ?mole glucose, g-1. h-1. The activation energy of the reaction was 49 kJ. mole-1. A proportion of the activity was highly resistant to storage at various temperatures: at 50 C 1,3-Beta-glucanase had a half-life of 28 days. © 1978.
Details
- Title
- Assay and properties of 1,3-Beta-glucanase in soil
- Authors
- G Lethbridge (Author) - University of Kent, United KingdomA T Bull (Author) - University of Kent, United KingdomRichard G Burns (Author) - University of Kent, United Kingdom
- Publication details
- Soil Biology and Biochemistry, Vol.10(5), pp.389-391
- Publisher
- Pergamon
- Date published
- 1978
- DOI
- 10.1016/0038-0717(78)90063-9
- ISSN
- 0038-0717
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
- Language
- English
- Record Identifier
- 99449331902621
- Output Type
- Journal article
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