Journal article
Aquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptide
Frontiers in Genetics, Vol.10, pp.1-14
2019
PMCID: PMC6389678
PMID: 30838021
Abstract
Wild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea cucumber reproductive processes. The neurohormone relaxin-like gonad-stimulating peptide (RGP) has been identified as the active gonad-stimulating peptide in sea stars (Asteroidea), which could also be present in other echinoderm groups. In this study, a sea cucumber RGP was identified and confirmed by phylogenetic analysis. A recombinant Holothuria scabra RGP was produced in the yeast Pichia pastoris and confirmed by mass spectrometry. To assess bioactivity, four levels of purification were tested in an in vitro germinal vesicle breakdown (GVBD) bioassay. The most pure form induced 98.56±1.19% GVBD in H. scabra and 89.57±1.19% GVBD in Holothuria leucospilota. Cruder levels of purification still resulted in some GVBD. Upon single injection into female H. scabra, the recombinant RGP induced head waving behavior followed by spawning within 90-170min. Spawned oocytes were fertilized successfully, larvae settled and developed into juveniles. Our results provide a key finding for the development of a break-through new artificial breeding approach in sea cucumber aquaculture.
Details
- Title
- Aquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptide
- Authors
- Chieu Hoang Dinh (Author) - University of the Sunshine Coast - School of Science & EngineeringLuke Turner (Author) - Tasmanian Seafoods Pty LtdMeaghan Smith (Author) - University of the Sunshine Coast - School of Science & EngineeringTianfang Wang (Author) - University of the Sunshine Coast - School of Science & EngineeringJosephine N Nocillado (Author) - University of the Sunshine Coast - School of Science & EngineeringPeter Palma (Author) - University of the Sunshine Coast - School of Science & EngineeringSaowaros Suwansa-ard (Author) - University of the Sunshine Coast - School of Science & EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - School of Science & EngineeringScott F Cummins (Author) - University of the Sunshine Coast - School of Science & Engineering
- Publication details
- Frontiers in Genetics, Vol.10, pp.1-14
- Publisher
- Frontiers Research Foundation
- Date published
- 2019
- DOI
- 10.3389/fgene.2019.00077
- ISSN
- 1664-8021
- PMID
- 30838021; PMC6389678
- Copyright note
- Copyright © 2019 Chieu, Turner, Smith, Wang, Nocillado, Palma, Suwansa-ard, Elizur and Cummins. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450655902621
- Output Type
- Journal article
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