Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes

A Numnuam; K Y Chumbimuni-Torres; Y Xiang; R Bash; P Thavarungkul; P Kanatharana; E Pretsch; J Wang; Eric Bakker

doi:10.1021/ac701910r

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Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes

Journal article

Peer reviewed

Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes

A Numnuam, K Y Chumbimuni-Torres, Y Xiang, R Bash, P Thavarungkul, P Kanatharana, E Pretsch, J Wang and Eric Bakker

Analytical Chemistry, Vol.80(3), pp.707-712

2008

DOI: https://doi.org/10.1021/ac701910r

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Abstract

electrochemical detection

detection limit

nucleic-acid

We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd(2+)-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 mu L microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 mu L samples with a lower detection limit of 0.14 nM corresponding: to 28 fmol of analyte. The results show great promise foil the potentiometric determination of proteins at very low concentrations in microliter samples.

Details

Title: Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes
Authors: A Numnuam (Author) - Purdue University, United States
K Y Chumbimuni-Torres (Author) - Purdue University, United States
Y Xiang (Author) - Arizona State University, United States
R Bash (Author) - Arizona State University, United States
P Thavarungkul (Author) - Prince of Songkla University, Thailand
P Kanatharana (Author) - Prince of Songkla University, Thailand
E Pretsch (Author) - ETH Zu¨rich, Switzerland
J Wang (Author) - Arizona State University, United States
Eric Bakker (Author) - Curtin University of Technology
Publication details: Analytical Chemistry, Vol.80(3), pp.707-712
Publisher: American Chemical Society
Date published: 2008
DOI: 10.1021/ac701910r
ISSN: 0003-2700
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450112502621
Output Type: Journal article

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Web Of Science research areas: Chemistry, Analytical

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