Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae

Tuan Son Le; Paul Southgate; Wayne O'Connor; Van Sang Vu; Ipek Kurtboke

doi:10.3390/antibiotics9070415

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Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae

Journal article

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Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae

Tuan Son Le, Paul Southgate, Wayne O'Connor, Van Sang Vu and Ipek Kurtboke

Antibiotics, Vol.9(7), pp.1-13

2020

DOI: https://doi.org/10.3390/antibiotics9070415

PMCID: PMC7400271

PMID: 32708768

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Abstract

Vibrio alginolyticus

vibriosis

bacteriophage

biocontrol

oyster larvae

Mortalities of bivalve larvae and spat linked with Vibrio spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing Vibrio spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic Vibrio isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the Myoviridae viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster (Saccostrea glomerata) larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries.

Details

Title: Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
Authors: Tuan Son Le (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Paul Southgate (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Wayne O'Connor (Author) - Port Stephens Fisheries Institute
Van Sang Vu (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Ipek Kurtboke (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Publication details: Antibiotics, Vol.9(7), pp.1-13
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/antibiotics9070415
PMID: 32708768; PMC7400271
Copyright note: Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Science and Engineering - Legacy; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99467308102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Pharmacology & Pharmacy