Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone

W M Huston; S Gloeckl; L de Boer; K W Beagley; Peter Timms

doi:10.1111/j.1600-0897.2010.00936.x

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Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone

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Peer reviewed

Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone

W M Huston, S Gloeckl, L de Boer, K W Beagley and Peter Timms

American Journal of Reproductive Immunology, Vol.65(5), pp.460-465

2011

DOI: https://doi.org/10.1111/j.1600-0897.2010.00936.x

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Abstract

Chlamydia

interferon regulatory factor

NFkappa B

wedelolactone

Problem Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability. Method of study Innate immune activation of HEp-2 cells during Chlamydia infection was conducted using lipopolysaccharide (LPS), polyI:C, and wedelolactone (innate immune inhibitor) to investigate the impact of these conditions on viability of Chlamydia. Results The addition of LPS and polyI:C to stimulate activation of the two distinct innate immune pathways (nuclear factor kappa beta and interferon regulatory factor) had no impact on the viability of Chlamydia. However, when compounds targeting either pathway were added in combination with the specific innate immune inhibitor (wedelolactone) a major impact on Chlamydia viability was observed. This impact was found to be due to the induction of apoptosis of the HEp-2 cells under these conditions. Conclusion This is the first time that induction of apoptosis has been reported in C. trachomatis-infected cells when treated with a combination of innate immune activators and wedelolactone. © 2010 John Wiley and Sons A/S.

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Title: Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone
Authors: W M Huston (Author) - Queensland University of Technology
S Gloeckl (Author) - Queensland University of Technology
L de Boer (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: American Journal of Reproductive Immunology, Vol.65(5), pp.460-465
Publisher: Wiley-Blackwell Publishing Inc.
Date published: 2011
DOI: 10.1111/j.1600-0897.2010.00936.x
ISSN: 1046-7408
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449102902621
Output Type: Journal article

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