Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction

Scott F Cummins; A E Nichols; C J Warso; G T Nagle

doi:10.1016/j.peptides.2004.10.024

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Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction

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Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction

Scott F Cummins, A E Nichols, C J Warso and G T Nagle

Peptides, Vol.26(3), pp.351-359

2005

DOI: https://doi.org/10.1016/j.peptides.2004.10.024

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Abstract

Fisheries Sciences

Aplysia

protein pheromone

seductin

attractin

enticin

temptin

Mate attraction in Aplysia involves the long-distance water-borne protein pheromones attractin, enticin, and temptin which are released during egg-laying. Other water-borne pheromones are predicted to act in concert with attractin, enticin, and temptin, but their identities were unknown. We recently identified a highly expressed Aplysia californica albumen gland gene (Alb-23) that encoded a novel protein by differential library screening of an albumen gland cDNA library. To determine whether Alb-23 ('seductin') was a water-borne pheromone, we employed Western blot analysis, purification and expression of albumen gland proteins, immunolocalization studies, pheromone secretion assays, comparative genomics, and behavioral bioassays. Immunoreactive seductin was detected in eluates of egg cordons, indicating that seductin was secreted onto the cordon during egg laying. Aplysia brasiliana seductin was 94% identical to its A. californica homolog. In T-maze attraction assays, the combination of attractin and seductin was significantly attractive to potential mates, whereas either protein alone was not. Data from this and previous studies support the hypothesis that seductin is a water-borne protein pheromone that acts in concert with attractin, enticin, and temptin to attract Aplysia to form and maintain mating aggregations.

Details

Title: Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction
Authors: Scott F Cummins (Author) - University of Texas, United States
A E Nichols (Author) - University of Texas, United States
C J Warso (Author) - Millersville University of Pennsylvania, United States
G T Nagle (Author) - University of Texas, United States
Publication details: Peptides, Vol.26(3), pp.351-359
Publisher: Elsevier Inc.
Date published: 2005
DOI: 10.1016/j.peptides.2004.10.024
ISSN: 0196-9781
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449704202621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy