Journal article
Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia
Journal of Medical Microbiology, Vol.60(11), pp.1633-1642
2011
Abstract
We studied 137 uropathogenic Escherichia coli (UPEC), isolated from hospitalized adult patients (Queensland, Australia), for their resistance to 17 antimicrobial agents using the Calibrated Dichotomous Sensitivity (CDS) method and the presence of classes l, ll and lll integron-associated integrases (intI genes) including functional class II intI2 as well as sul1, sul2 and sul3 genes using PCR. Randomly amplified polymorphic DNA PCR, a high resolution PhP-fingerprinting method and phylogenetic grouping were also used to identify the clonality of the sulphafurazole resistant isolates. One hundred and twenty (87.6%) isolates were resistance to one or more of the tested antimicrobial drugs with the highest resistance (70%) observed against sulphafurazole (96 isolates). Of these, 84 (87.5%) contained one or more sul alleles with sul1 gene being the most common allele occurring in 69 (72%) isolates. Only 38 out of 69 (55%) strains carrying sul1 gene, were positive for class l integrase. Our results indicate a high prevalence of sulphafurazole resistant UPEC strains belonging to different clones among patients with UTI in Queensland. We also conclude that these strains carry predominantly sul1 gene that is not commonly associated with presence of class l integrase indicating that it may be carried on either bacterial chromosome or other genetic elements.
Details
- Title
- Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia
- Authors
- Aycan Gundogdu (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationYsanne B Long (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationTara L Vollmerhausen (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- Journal of Medical Microbiology, Vol.60(11), pp.1633-1642
- Publisher
- Society for General Microbiology
- Date published
- 2011
- DOI
- 10.1099/jmm.0.034140-0
- ISSN
- 0022-2615; 0022-2615
- Copyright note
- Copyright © 2011 Society for General Microbiology. The accepted manuscript is reproduced in accordance with the copyright policy of the publisher.
- Organisation Unit
- School of Science and Engineering - Legacy; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449527702621
- Output Type
- Journal article
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