Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)

A Kollipara; C Wan; G Rawlinson; J Brumm; K Nilsson; Adam Polkinghorne; K W Beagley; Peter Timms

doi:10.1016/j.vaccine.2012.12.057

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Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)

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Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)

A Kollipara, C Wan, G Rawlinson, J Brumm, K Nilsson, Adam Polkinghorne, K W Beagley and Peter Timms

Vaccine, Vol.31(8), pp.1217-1223

2013

DOI: https://doi.org/10.1016/j.vaccine.2012.12.057

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Abstract

Chlamydia pecorum

Koala

vaccine

MOMP

Chlamydia continues to be a major pathogen of koalas. The bacterium is associated with ocular, respiratory and urogenital tract infections and a vaccine is considered the best option to limit the decline of mainland koala populations. Over the last 20 years, efforts to develop a chlamydial vaccine in humans have focussed on the use of the chlamydial major outer membrane protein (MOMP). Potential problems with the use of MOMP-based vaccines relate to the wide range of genetic diversity in its four variable domains. In the present study, we evaluated the immune response of koalas vaccinated with a MOMP-based C. pecorum vaccine formulated with genetically and serologically diverse MOMPs. Animals immunised with individual MOMPs developed strong antibody and lymphocyte proliferation responses to both homologous as well as heterologous MOMP proteins. Importantly, we also showed that vaccine induced antibodies which effectively neutralised various heterologous strains of koala C. pecorum in an in vitro assay. Finally, we also demonstrated that the immune responses in monovalent as well as polyvalent MOMP vaccine groups were able to recognise whole chlamydial elementary bodies, illustrating the feasibility of developing an effective MOMP based C. pecorum vaccine that could protect against a range of strains. © 2013.

Details

Title: Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)
Authors: A Kollipara (Author) - Queensland University of Technology
C Wan (Author) - Queensland University of Technology
G Rawlinson (Author) - Lone Pine Koala Sanctuary
J Brumm (Author) - Lone Pine Koala Sanctuary
K Nilsson (Author) - Lone Pine Koala Sanctuary
Adam Polkinghorne (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: Vaccine, Vol.31(8), pp.1217-1223
Publisher: Elsevier Ltd.
Date published: 2013
DOI: 10.1016/j.vaccine.2012.12.057
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449053702621
Output Type: Journal article

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