Files and links (1)
Published VersionCC BY V4.0, Open Access
Journal article
Anticancer Effects of Sacha Inchi ( Plukenetia volubilis ) Shell Extract on Colon Cancer Cells: Integrated GC-MS, LC-MS, Transcriptomic, and Proteomic Analyses
International Journal of Molecular Sciences, Vol.27(1), pp.1-25
2026
PMID: 41516111
Abstract
With an aging population and the increase in life expectancy, colorectal cancer is becoming increasingly common. Currently, the mainstay of treatment is chemotherapy, which can cause a variety of side effects. However, herbalists in many cultures have long used herbs to promote health and treat various diseases, including colon cancer. This study investigated the chemical components of shell extracts from
(sacha inchi) and their effects on colon cancer cells. First, cytotoxicity against both normal and cancer cells was assessed, followed by cell migration and invasion. Finally, transcriptomic and proteomic analyses were performed to investigate molecular mechanisms. Phytochemical analysis showed a total phenolic content of 134.61 ± 0.27 mgGAE/g extract and a total flavonoid content of 5.75 ± 0.01 mgQE/g extract; moreover, lidocaine (10.57%) and linolenic acid (10.39%) were identified as the most abundant compounds. In vitro, the extract inhibited cell migration, invasion, and colony formation and was associated with potential modulations of the Hippo pathways and epithelial-mesenchymal transition. Therefore, it can be concluded that these extracts are effective in inhibiting the progression of colon cancer cells, and thus, they can prospectively be developed as a dietary supplement or therapeutic agent for the treatment of colon cancer in the future.
Details
- Title
- Anticancer Effects of Sacha Inchi ( Plukenetia volubilis ) Shell Extract on Colon Cancer Cells: Integrated GC-MS, LC-MS, Transcriptomic, and Proteomic Analyses
- Authors
- Supawadee Osotprasit - Burapha UniversitySaowaros Suwansa-Ard - University of the Sunshine Coast, Queensland, Centre for BioinnovationScott F Cummins - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang - University of the Sunshine Coast, Queensland, Centre for BioinnovationStuart J Smith - University of the Sunshine Coast, Queensland, Centre for BioinnovationTepparit Samrit - Burapha UniversityAthit Chaiwichien - Burapha UniversityNarin Changklungmoa - Burapha UniversityPornanan Kueakhai (Corresponding Author) - Burapha University
- Publication details
- International Journal of Molecular Sciences, Vol.27(1), pp.1-25
- Publisher
- MDPI AG
- Date published
- 2026
- DOI
- 10.3390/ijms27010234
- ISSN
- 1422-0067; 1661-6596
- PMID
- 41516111
- Copyright note
- © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
- Data Availability
- The datasets generated and analyzed during the current study are publicly available. The raw data from whole transcriptome sequencing are deposited under the BioProject accession number PRJNA1236814 in both the NCBI BioProject and SRA databases, accessible at https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1236814 (accessed on 16 March 2025). Raw data from peptide mass spectrometry are accessible under the accession number PXD062512 at https://www.ebi.ac.uk/pride/archive/projects/PXD062512 in the PRIDE database (accessed on 11 November 2025).
- Grant note
- This work was supported by the National Research Council of Thailand (NRCT; N41A650402) and TAI.C.M.S. Standard industrial company limited.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991198446702621
- Output Type
- Journal article
Metrics
2 Record Views