Journal article
Antibody and Cytokine Responses of Koalas (Phascolarctos cinereus) Vaccinated with Recombinant Chlamydial Major Outer Membrane Protein (MOMP) with Two Different Adjuvants
PLoS One, Vol.11(5), e0156094
2016
Abstract
Developing a vaccine against Chlamydia is key to combating widespread mortalities and morbidities associated with this infection in koalas (Phascolarctos cinereus). In previous studies, we have shown that two or three doses of a Recombinant Major Outer Membrane Protein (rMOMP) antigen-based vaccine, combined with immune stimulating complex (ISC) adjuvant, results in strong cellular and humoral immune responses in koalas. We have also separately evaluated a single dose vaccine, utilising a tri-adjuvant formula that comprises polyphosphazine based poly I: C and host defense peptides, with the same antigen. This formulation also produced strong cellular and humoral immune responses in captive koalas. In this current study, we directly compared the host immune responses of two sub-groups of wild Chlamydia negative koalas in one population vaccinated with the rMOMP protein antigen and adjuvanted with either the ISC or tri-adjuvant formula. Overall, both adjuvants produced strong Chlamydia-specific cellular (IFN-γ and IL-17A) responses in circulating PBMCs as well as MOMP-specific and functional, in vitro neutralising antibodies. While the immune responses were similar, there were adjuvant-specific immune differences between the two adjuvants, particularly in relation to the specificity of the MOMP epitope antibody responses.
Details
- Title
- Antibody and Cytokine Responses of Koalas (Phascolarctos cinereus) Vaccinated with Recombinant Chlamydial Major Outer Membrane Protein (MOMP) with Two Different Adjuvants
- Authors
- Shahneaz Khan (Author) - Queensland University of TechnologyMarion Desclozeaux (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringCourtney Waugh (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJon Hanger (Author) - Endeavour Veterinary Ecology Pty LtdJo Loader (Author) - Endeavour Veterinary Ecology Pty LtdVolker Gerdts (Author) - University of Saskatchewan, CanadaAndrew Potter (Author) - University of Saskatchewan, CanadaAdam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringKenneth Beagley (Author) - Queensland University of TechnologyPeter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- PLoS One, Vol.11(5), e0156094; 17
- Publisher
- Public Library of Science
- Date published
- 2016
- DOI
- 10.1371/journal.pone.0156094
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2016 Khan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Science and Engineering - Legacy; School of Education and Tertiary Access; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449469802621
- Output Type
- Journal article
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