Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection

Melanie Rampton; Shelley F Walton; D C Holt; C Passay; A Kelly; B J Currie; J S McCarthy; Kate E Mounsey

doi:10.1371/journal.pone.0065354

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Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection

Journal article

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Peer reviewed

Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection

Melanie Rampton, Shelley F Walton, D C Holt, C Passay, A Kelly, B J Currie, J S McCarthy and Kate E Mounsey

PLoS One, Vol.8(6), e65354

2013

DOI: https://doi.org/10.1371/journal.pone.0065354

PMCID: PMC3675102

PMID: 23762351

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Abstract

Sarcoptes

antibody

No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

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Title: Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection
Authors: Melanie Rampton (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Shelley F Walton (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
D C Holt (Author) - Charles Darwin University
C Passay (Author) - Queensland Institute of Medical Research
A Kelly (Author) - University of Queensland
B J Currie (Author) - Charles Darwin University
J S McCarthy (Author) - Queensland Institute of Medical Research
Kate E Mounsey (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: PLoS One, Vol.8(6), e65354
Publisher: Public Library of Science
Date published: 2013
DOI: 10.1371/journal.pone.0065354
ISSN: 1932-6203
PMID: 23762351; PMC3675102
Copyright note: Copyright © 2013 Rampton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Organisation Unit: School of Health - Biomedicine; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99448799302621
Output Type: Journal article

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Web Of Science research areas: Veterinary Sciences

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