Journal article
Angelica sinensis polysaccharide protects against acetaminophen-induced acute liver injury and cell death by suppressing oxidative stress and hepatic apoptosis in vivo and in vitro
International Journal of Biological Macromolecules, Vol.111, pp.1133-1139
2018
PMID: 29415408
Abstract
Acetaminophen (APAP)-induced hepatic damage is prevalent in western countries. The present study aimed to investigate the hepatoprotective effects of Angelica sinensis polysaccharide (ASP), an active constituent derived from a water extract of Angelica sinensis, in rats exposed to an APAP overdose. The mechanisms underlying the activity of this compound were also considered. Specifically, serum and hepatic biochemical parameters including alanine aminotransferase (ALT), aspartate transaminase (AST), glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) were evaluated, and key proteins involved in hepatic apoptosis, including cleaved caspase-3, Bax and Bcl-2 were quantified. In vivo, H&E staining reveals that ASP reduces the degeneration of hepatocytes and the amount of cytoplasmic vacuolation in rats exposed to an overdose of APAP. ASP markedly alleviated liver injury via an increase in GSH levels and the inhibition of hepatic apoptosis. In vitro, ASP significantly elevated the survival rate of rat primary hepatocytes exposed to an overdose of APAP. The beneficial effect might be, at least in part, due to the amelioration of lipid peroxidation and oxidative stress, along with the inhibition of apoptosis. Taken together, our findings reveal that ASP has potential to be used as a hepatoprotective agent for the management of APAP-induced liver injury.
Details
- Title
- Angelica sinensis polysaccharide protects against acetaminophen-induced acute liver injury and cell death by suppressing oxidative stress and hepatic apoptosis in vivo and in vitro
- Authors
- Peng Cao - Huazhong University of Science and TechnologyJinlu Sun - Huazhong University of Science and TechnologyMitchell A Sullivan - Translational Research InstituteXiao Huang - Huazhong University of Science and TechnologyHanxiang Wang - Huazhong University of Science and TechnologyYu Zhang - Huazhong University of Science and TechnologyNa Wang - Renmin Hospital of Wuhan UniversityKaiping Wang (Corresponding Author) - Huazhong University of Science and Technology
- Publication details
- International Journal of Biological Macromolecules, Vol.111, pp.1133-1139
- Publisher
- Elsevier BV
- Date published
- 2018
- DOI
- 10.1016/j.ijbiomac.2018.01.139
- ISSN
- 1879-0003
- PMID
- 29415408
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035096402621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Applied
- Polymer Science
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Source: InCites