Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii

Thanapong Kruangkum; Kornchanok Jaiboon; Phakkhananan Pakawanit; Jirawat Saetan; Arnon Pudgerd; Suttipong Wannapaiboon; Charoonroj Chotwiwatthanakun; Scott F Cummins; Prasert Sobhon; Rapeepun Vanichviriyakit

doi:10.1007/s00441-024-03898-3

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Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii

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Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii

Thanapong Kruangkum, Kornchanok Jaiboon, Phakkhananan Pakawanit, Jirawat Saetan, Arnon Pudgerd, Suttipong Wannapaiboon, Charoonroj Chotwiwatthanakun, Scott F Cummins, Prasert Sobhon and Rapeepun Vanichviriyakit

Cell and Tissue Research, Vol.397, pp.125-146

2024

DOI: https://doi.org/10.1007/s00441-024-03898-3

PMCID: PMC11291661

PMID: 38878176

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Abstract

crustacean

nephopore

kidney

bladder

neurohormones

In this study, the complex organization of the AnG in the giant freshwater prawn Macrobrachium rosenbergii was revealed using various techniques, including conventional histology, histochemistry, scanning electron microscopy, and X-ray tomography. The results showed the diversity of cells in the AnG and the detailed organization of the labyrinth's tubule into four radiated areas from the central to peripheral zones. The study also demonstrated the expression of some vertebrate kidney-associated homolog genes, aquaporin (AQP), solute carrier family 22 (SLC-22), nephrin, and uromodulin, in the AnG by qPCR. The result of in situ hybridization further showed the localization of SLC-22 and AQP transcript in the bladder and labyrinth's epithelium, specifically in regions 2, 3, and 4. Additionally, the study revealed neuropeptide expressions in the AnG by qPCR and in situ hybridization, i.e., crustacean hyperglycemic hormone (CHH) and molt inhibiting hormone (MIH), implying that the AnG may have a role in hormone production. Moreover, male and female prawns exhibited different levels of AQP, SLC-22, nephrin, and CHH expressions during the premolt and intermolt stages, suggesting a crucial role relevant to the molting stages. In conclusion, this study clarified the complex structure of the AnG in M. rosenbergii and demonstrated for the first time the expression of vertebrate kidney-associated genes and the possible endocrine role of the AnG. Further investigation is needed to clarify the role of these genes, particularly during ecdysis. The implications of these findings could significantly advance our understanding of the AnG in decapod crustaceans.

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Title: Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii
Authors: Thanapong Kruangkum - Mahidol University
Kornchanok Jaiboon - Mahidol University
Phakkhananan Pakawanit - Synchrotron Light Research Institute
Jirawat Saetan - Prince of Songkla University
Arnon Pudgerd - University of Phayao
Suttipong Wannapaiboon - Synchrotron Light Research Institute
Charoonroj Chotwiwatthanakun - Mahidol University
Scott F Cummins - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Prasert Sobhon - Mahidol University
Rapeepun Vanichviriyakit (Corresponding Author) - Mahidol University
Publication details: Cell and Tissue Research, Vol.397, pp.125-146
Publisher: Springer
Date published: 2024
DOI: 10.1007/s00441-024-03898-3
ISSN: 1432-0878
PMID: 38878176; PMC11291661
Copyright note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Data Availability: Data supporting this study are openly available from the AnG transcriptome database (SRA number: PRJNA381087), GenBank accession number AF219382.1, GenBank accession number KC990939.1, and included within the supplementary data.
Organisation Unit: School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 991039594102621
Output Type: Journal article

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Web Of Science research areas: Cell Biology

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