Analysis of the relationship between mannose-binding lectin (MBL) genotype, MBL levels and function in an Australian blood donor population

R M Minchinton; M M Dean; T R Clark; S Heatley; C G Mullighan

doi:10.1046/j.1365-3083.2002.01167.x

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Analysis of the relationship between mannose-binding lectin (MBL) genotype, MBL levels and function in an Australian blood donor population

Journal article

Peer reviewed

Analysis of the relationship between mannose-binding lectin (MBL) genotype, MBL levels and function in an Australian blood donor population

R M Minchinton, M M Dean, T R Clark, S Heatley and C G Mullighan

Scandinavian Journal of Immunology, Vol.56(6), pp.630-641

2002

DOI: https://doi.org/10.1046/j.1365-3083.2002.01167.x

PMID: 12472676

Abstract

The mannose-binding lectin (MBL) pathway of complement activation is an important component of innate host defence. Numerous studies have described associations between the MBL genotype, MBL levels and disease susceptibility. However, genotyping and quantitative assays used in these studies have frequently been limited, and comprehensive data examining the interaction between structural and coding MBL genetic variants, MBL antigenic levels and MBL functional activity are lacking. Such data may be important for accurate planning and interpretation of studies of MBL and disease. This study has examined MBL in a cohort of 236 Australian blood donors. Five MBL promoter and coding single nucleotide polymorphisms were genotyped using polymerase chain reaction-sequence-specific priming (PCR-SSP). Plasma levels of MBL antigen were quantified using a double-antibody enzyme-linked immunosorbent assay (ELISA), and functional MBL levels were quantified using a mannan-binding assay. Activation of the complement pathway by MBL was measured in a C4-deposition assay. Significant associations were found between both coding and promoter polymorphisms and MBL antigenic and functional levels. There was significant correlation between the results of MBL double-antibody, mannan-binding and C4-deposition assays. Comprehensive MBL genotyping and functional MBL quantitation using mannan-binding and C4-deposition assays have the potential to be highly informative in MBL disease association studies.

Details

Title: Analysis of the relationship between mannose-binding lectin (MBL) genotype, MBL levels and function in an Australian blood donor population
Authors: R M Minchinton - Australian Red Cross Blood Service
M M Dean - Australian Red Cross Blood Service
T R Clark - Australian Red Cross Blood Service
S Heatley - Australian Red Cross Blood Service
C G Mullighan - South Australia Pathology
Publication details: Scandinavian Journal of Immunology, Vol.56(6), pp.630-641
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2002
DOI: 10.1046/j.1365-3083.2002.01167.x
ISSN: 1365-3083
PMID: 12472676
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991037506202621
Output Type: Journal article