Analysis of cytoskeletal features in dried and living cells and slow dynamic experiments using atomic force microscopy

Gregory S Watson; Jolanta A Watson

doi:10.1088/1742-6596/100/5/052041

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Analysis of cytoskeletal features in dried and living cells and slow dynamic experiments using atomic force microscopy

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Analysis of cytoskeletal features in dried and living cells and slow dynamic experiments using atomic force microscopy

Gregory S Watson and Jolanta A Watson

Journal of Physics: Conference Series, Vol.100(Part 5), pp.1-4

2008

DOI: https://doi.org/10.1088/1742-6596/100/5/052041

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Abstract

atomic force microscopy

The ability to interact with live cells in vitro in combination with either sequential image acquisition or continuous force sensing provide the means for tracking the dynamics of intra- or inter-cell processes. The former operational mode is relatively slow and is most suitable for events where the temporal evolution takes place on a time scale of several minutes to hours. For instance, aspects of cytoskeletal dynamics, cell motility or conformational response to external stimuli fall into this category. In this study we demonstrate cell responses from exposure to cytochalasin, glutaraldehyde and a tetrazolium salt. Significant changes in mechanical properties and structural features are observed on time scales up to 3 hrs. In addition, comparative studies of cytoskeletal components of dried and living cells are undertaken. © 2008 IOP Publishing Ltd.

Details

Title: Analysis of cytoskeletal features in dried and living cells and slow dynamic experiments using atomic force microscopy
Authors: Gregory S Watson (Author) - Griffith University
Jolanta A Watson (Author) - Griffith University
Publication details: Journal of Physics: Conference Series, Vol.100(Part 5), pp.1-4
Publisher: Institute of Physics Publishing Ltd.
Date published: 2008
DOI: 10.1088/1742-6596/100/5/052041
ISSN: 1742-6588
Copyright note: Copyright © 2008. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Published under licence by IOP Publishing Ltd
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449370902621
Output Type: Journal article

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Web Of Science research areas: Materials Science, Coatings & Films; Nanoscience & Nanotechnology; Physics, Applied