Analysis of collagen fibril diameter distribution in connective tissues using small-angle X-ray scattering

Kheng Lim Goh; Jennifer C Hiller; J Louise Haston; David F Holmes; Karl E Kadler; Ann Murdoch; Judith R Meakin; Tim J Wess

doi:10.1016/j.bbagen.2004.12.004

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Analysis of collagen fibril diameter distribution in connective tissues using small-angle X-ray scattering

Journal article

Peer reviewed

Analysis of collagen fibril diameter distribution in connective tissues using small-angle X-ray scattering

Kheng Lim Goh, Jennifer C Hiller, J Louise Haston, David F Holmes, Karl E Kadler, Ann Murdoch, Judith R Meakin and Tim J Wess

Biochimica et Biophysica Acta - General Subjects, Vol.1722(2), pp.183-188

2005

DOI: https://doi.org/10.1016/j.bbagen.2004.12.004

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Abstract

collagen fibril diameter

scanning transmission electron microscopy

small-angle X-ray scattering

simulated annealing

Analysis of the diameters of collagen fibrils provides insight into the structure and physical processes occurring in the tissue. This paper describes a method for analyzing the frequency distribution of the diameters of collagen fibrils from small-angle X-ray scattering (SAXS) patterns. Frequency values of fibril diameters were input into a mathematical model of the form factor to calculate the equatorial intensity which best fits the experimentally derived data from SAXS patterns. A minimization algorithm utilizing simulated annealing (SA) was used in the fitting procedure. The SA algorithm allowed for random sampling of the frequency values, and was run iteratively to build up an optimized frequency distribution of fibril diameters. Results were obtained for collagen samples from sheep spine ligaments. The mean fibril diameter value obtained from this data-fitting method was 73 nm±20 nm (S.D.). From scanning transmission electron microscopy, the mean diameter was found to be 69 nm±14 nm (S.D.). The good agreement between the two methods demonstrates the reliability of the SAXS method for the tissue examined. The non-destructive nature of this technique, as well as its statistical robusticity and capacity for large sampling, means that this method is both quick and effective. © 2004 Elsevier B.V. All rights reserved.

Details

Title: Analysis of collagen fibril diameter distribution in connective tissues using small-angle X-ray scattering
Authors: Kheng Lim Goh (Author) - Cardiff University, United Kingdom
Jennifer C Hiller (Author) - Cardiff University, United Kingdom
J Louise Haston (Author) - Stirling University, United Kingdom
David F Holmes (Author) - University of Manchester, United Kingdom
Karl E Kadler (Author) - University of Manchester, United Kingdom
Ann Murdoch (Author) - Aberdeen University, United Kingdom
Judith R Meakin (Author) - Aberdeen University, United Kingdom
Tim J Wess (Author) - Cardiff University, United Kingdom
Publication details: Biochimica et Biophysica Acta - General Subjects, Vol.1722(2), pp.183-188
Publisher: Elsevier BV
Date published: 2005
DOI: 10.1016/j.bbagen.2004.12.004
ISSN: 0304-4165
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450709802621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics