An investigation of bioactive phytochemicals in the leaves of Melicope vitiflora by electrospray ionisation ion trap mass spectrometry

F O'Donnell; V N Ramachandran; T J Smyth; W F Smyth; Peter R Brooks

doi:10.1016/j.aca.2008.11.066

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An investigation of bioactive phytochemicals in the leaves of Melicope vitiflora by electrospray ionisation ion trap mass spectrometry

Journal article

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An investigation of bioactive phytochemicals in the leaves of Melicope vitiflora by electrospray ionisation ion trap mass spectrometry

F O'Donnell, V N Ramachandran, T J Smyth, W F Smyth and Peter R Brooks

Analytica Chimica Acta, Vol.634(1), pp.115-120

2009

DOI: https://doi.org/10.1016/j.aca.2008.11.066

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Abstract

bioactive phytochemicals

Melicope vitiflora

electrospray ionisation ion trap mass spectrometry

The ethyl acetate extract of the leaves of Melicope vitiflora was separated by column chromatography and the resulting fractions tested for their bioactivity towards methicillin-resistant-Staphylococcus aureus (MRSA) and Micrococcus luteus (ML). The bioactive column chromatography fractions were further separated by preparative TLC and dereplication was carried out on them by first subjecting them to electrospray ionisation-ion trap mass spectrometry (ESI-MSn). The resulting molecular masses, their fragmentation patterns in addition to the chemnet database (www.chemnetbase.com) were used to aid in the structural elucidation of some of the compounds by permitting comparison with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multi drug resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for M. vitiflora.

Details

Title: An investigation of bioactive phytochemicals in the leaves of Melicope vitiflora by electrospray ionisation ion trap mass spectrometry
Authors: F O'Donnell (Author) - University of Ulster, United Kingdom
V N Ramachandran (Author) - University of Ulster, United Kingdom
T J Smyth (Author) - University of Ulster, United Kingdom
W F Smyth (Author) - University of Ulster, United Kingdom
Peter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Analytica Chimica Acta, Vol.634(1), pp.115-120
Publisher: Elsevier BV
Date published: 2009
DOI: 10.1016/j.aca.2008.11.066
ISSN: 0003-2670
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449220702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Chemistry, Analytical

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