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An international review of the characteristics of viral nucleic acid-amplification testing (NAT) reveals a trend towards the use of smaller pool sizes and individual donation NAT
Journal article   Open access   Peer reviewed

An international review of the characteristics of viral nucleic acid-amplification testing (NAT) reveals a trend towards the use of smaller pool sizes and individual donation NAT

Helen M Faddy, Carla Osiowy, Brian Custer, Michael Busch, Susan L Stramer, Melinda M Dean, Jessika Acutt, Elvina Viennet, Thijs van de Laar, Wai-Chiu Tsoi, …
Vox Sanguinis, Vol.119(7), pp.745-751
2024
PMID: 38516962
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Vox Sanguinis - 2024 - Faddy - An international review of the characteristics of viral nucleic acid‐amplification testing 1.76 MBDownloadView
Published Version Open Access CC BY-NC-ND V4.0

Abstract

NAT transfusion safety virus
Background and Objectives Nucleic acid-amplification testing (NAT) is used for screening blood donations/donors for blood-borne viruses. We reviewed global viral NAT characteristics and NAT-yield confirmatory testing used by blood operators. Materials and Methods NAT characteristics and NAT-yield confirmatory testing used during 2019 was surveyed internationally by the International Society of Blood Transfusion Working Party Transfusion-Transmitted Infectious Diseases. Reported characteristics are presented herein. Results NAT was mainly performed under government mandate. Human immunodeficiency virus (HIV), hepatitis C virus (HCV) and hepatitis B virus (HBV) NAT was performed on all donors and donation types, while selective testing was reported for West Nile virus, hepatitis E virus (HEV), and Zika virus. Individual donation NAT was used for HIV, HCV and HBV by ~50% of responders, while HEV was screened in mini-pools by 83% of responders performing HEV NAT. Confirmatory testing for NAT-yield samples was generally performed by NAT on a sample from the same donation or by NAT and serology on samples from the same donation and a follow-up sample. Conclusion In the last decade, there has been a trend towards use of smaller pool sizes or individual donation NAT. We captured characteristics of NAT internationally in 2019 and provide insights into confirmatory testing approaches used for NAT-yields, potentially benefitting blood operators seeking to implement NAT.

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