An analysis of the lamellar structure of sea urchin egg cortical granules using X-ray scattering

T Whalley; J G Grossmann; Tim J Wess

doi:10.1016/S0014-5793(00)02068-8

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An analysis of the lamellar structure of sea urchin egg cortical granules using X-ray scattering

Journal article

Peer reviewed

An analysis of the lamellar structure of sea urchin egg cortical granules using X-ray scattering

T Whalley, J G Grossmann and Tim J Wess

FEBS Letters, Vol.482(3), pp.242-246

2000

DOI: https://doi.org/10.1016/S0014-5793(00)02068-8

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Abstract

cortical granule

molecular packing

proteoglycan

Cortical granules (CGs) are secretory vesicles associated with egg and oocyte plasma membranes that undergo exocytosis at fertilisation. In the sea urchin Strongylocentrotus purpuratus, the internal organisation of these CGs exhibits a lamellar-type morphology. The different lamellar layers correspond to proteoglycans, structural proteins and enzymes required for fertilisation envelope assembly and modification of the post-fertilisation egg surface. We have studied the lamellar structure of CGs using X-ray scattering and reveal the contrast density variation of the lamellae in the native state. The structure of functionally competent CGs in situ differs significantly from that determined by electron microscopic studies. We observed a strong periodicity of the lamellar structure of 280 Ã… as opposed to the 590 Ã… repeat observed previously. Fusion of the CGs produced a loss of the lamellar repeat and the development of a broad peak corresponding to a 20 Ã… periodicity that may be indicative of the molecular packing in the resulting hydrated gel structure. (C) 2000 Federation of European Biochemical Societies.

Details

Title: An analysis of the lamellar structure of sea urchin egg cortical granules using X-ray scattering
Authors: T Whalley (Author) - University of Stirling, United Kingdom
J G Grossmann (Author) - CLRC Daresbury Laboratory, United Kingdom
Tim J Wess (Author) - University of Stirling, United Kingdom
Publication details: FEBS Letters, Vol.482(3), pp.242-246
Publisher: John Wiley & Sons Ltd.
Date published: 2000
DOI: 10.1016/S0014-5793(00)02068-8
ISSN: 0014-5793
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451248002621
Output Type: Journal article

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