Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

K Y Chumbimuni-Torres; Jie Wu; C Clawson; M Galik; A Walter; G Flechsig; Eric Bakker; L Zhang; J Wang

doi:10.1039/C0AN00198H

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Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

Journal article

Peer reviewed

Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

K Y Chumbimuni-Torres, Jie Wu, C Clawson, M Galik, A Walter, G Flechsig, Eric Bakker, L Zhang and J Wang

The Analyst, Vol.135(7), pp.1618-1623

2010

DOI: https://doi.org/10.1039/C0AN00198H

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Abstract

Amplified potentiometric transduction of DNA hybridization based on using liposome 'nanocarriers' loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of 'green' calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays.

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Title: Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes
Authors: K Y Chumbimuni-Torres (Author) - University of California, United States
Jie Wu (Author) - University of California, United States
C Clawson (Author) - University of California, United States
M Galik (Author) - University of California, United States
A Walter (Author) - University of Rostock, Germany
G Flechsig (Author) - University of Rostock, Germany
Eric Bakker (Author) - Curtin University of Technology
L Zhang (Author) - University of California, United States
J Wang (Author) - University of California, United States
Publication details: The Analyst, Vol.135(7), pp.1618-1623
Publisher: Royal Society of Chemistry (R S C) Publications
DOI: 10.1039/C0AN00198H
ISSN: 0003-2654
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450046502621
Output Type: Journal article

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