Amplification of the polymorphic 5.8S rRNA gene from selected Australian gigartinalean species (Rhodophyta) by polymerase chain reaction

Dorothy A Steane; B McClure; Adrienne E Clarke; G Kraft

doi:10.1111/j.0022-3646.1991.00758.x

Back

Amplification of the polymorphic 5.8S rRNA gene from selected Australian gigartinalean species (Rhodophyta) by polymerase chain reaction

Journal article

Peer reviewed

Amplification of the polymorphic 5.8S rRNA gene from selected Australian gigartinalean species (Rhodophyta) by polymerase chain reaction

Dorothy A Steane, B McClure, Adrienne E Clarke and G Kraft

Journal of Phycology, Vol.27(6), pp.758-762

1991

DOI: https://doi.org/10.1111/j.0022-3646.1991.00758.x

Files and links (1)

url

https://doi.org/10.1111/j.0022-3646.1991.00758.xView

Published Version

Abstract

Plant Biology

Australia

Gigartinales

polymerase chain reaction

rDNA

Rhodophyta

rRNA

We have initiated comparative studies of ribosomal RNA (rRNA) gene structure to explore its potential to provide taxonomically useful data within the large red algal order Gigartinales. In southern Australia, this group is extremely diverse and includes large numbers of endemic taxa, many of potential economic importance. The 5.8S rRNA gene occurs in the middle region of the ribosomal DNA (rDNA) cistron and is flanked by two internal transcribed spacers (ITSs). These spacers contain regions of DNA, which are highly consented at the generic level and above, interspersed with highly divergent sequences. The 5.8S and associated ITS s of 11 species of Gigartinales (including five species of the largest Australian endemic marine algal genus, Mychodea), plus five taxa belonging to other orders, were amplified by the polymerase chain reaction. The size of the 5.8S rDNA and its flanking ITSs varied not only within and between genera, but also at the species level. However, this rDNA sequence appears to be relatively constant within populations find may be useful as a populational marker.

Details

Title: Amplification of the polymorphic 5.8S rRNA gene from selected Australian gigartinalean species (Rhodophyta) by polymerase chain reaction
Authors: Dorothy A Steane (Author) - University of Tasmania
B McClure (Author) - University of Melbourne
Adrienne E Clarke (Author) - University of Melbourne
G Kraft (Author) - University of Melbourne
Publication details: Journal of Phycology, Vol.27(6), pp.758-762
Publisher: Wiley-Blackwell Publishing Inc.
Date published: 1991
DOI: 10.1111/j.0022-3646.1991.00758.x
ISSN: 0022-3646
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450221102621
Output Type: Journal article

Metrics

4 File views/ downloads

2197 Record Views

45 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Marine & Freshwater Biology; Plant Sciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites