Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide

C. McDermott; R. Chess-Williams; K.A. Mills; S.H. Kang; S.E. Farr; G.D. Grant; A.V. Perkins; A.K. Davey; S. Anoopkumar-Dukie

doi:10.1016/j.tiv.2013.04.015

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Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide

Journal article

Peer reviewed

Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide

C. McDermott, R. Chess-Williams, K.A. Mills, S.H. Kang, S.E. Farr, G.D. Grant, A.V. Perkins, A.K. Davey and S. Anoopkumar-Dukie

Toxicology in Vitro, Vol.27(6), pp.1693-1698

2013

DOI: https://doi.org/10.1016/j.tiv.2013.04.015

PMID: 23665401

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Abstract

Acetylcholine

Inflammation

Lipopolysaccharide

PGE2

Pyocyanin

Urothelium

The effects of pseudomonal virulence factor pyocyanin, and LPS from Pseudomonas aeruginosa and Escherichia coli on urothelial mediator release and cytokine production were examined. RT4 urothelial cells were treated with pyocyanin (1–100 μM) or LPS (1–100 ng/mL) for 24-h. Effects were measured in terms of changes in cell viability, basal and stretch-induced acetylcholine (Ach) and PGE2 release, and inflammatory cytokines (IL-6 and IL-12) production. Twenty-four hour pyocyanin (100 μM) treatment significantly decreased urothelial cell viability, while stretch-induced Ach release response was inhibited. E. coli LPS (100 ng/mL) produced a similar response with an additional significant increase in basal Ach release. All three virulence factors significantly increased urothelial PGE2 release; under basal release for pyocyanin (100 μM), stretch-induced release for pseudomonal LPS (⩾10 ng/mL) and both basal and stimulated release for E. coli LPS (⩾10 ng/mL). IL-6 and IL-12 were not detected in control samples, however 24 h treatment with pyocyanin (100 μM) or LPS (100 ng/mL) resulted in IL-6 release from urothelial cells. The changes in urothelial Ach and PGE2, and release of inflammatory cytokine IL-6 induced by exposure to the bacterial virulence factors may play a role in the symptoms of pain and urinary urgency experienced with urinary tract infections.

Details

Title: Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide
Authors: C. McDermott (Corresponding Author) - Bond University
R. Chess-Williams (Author) - Bond University
K.A. Mills (Author) - Bond University
S.H. Kang (Author) - Bond University
S.E. Farr (Author) - Bond University
G.D. Grant (Author) - Griffith University
A.V. Perkins (Author) - Griffith University
A.K. Davey (Author) - Griffith University
S. Anoopkumar-Dukie (Author) - Griffith University
Publication details: Toxicology in Vitro, Vol.27(6), pp.1693-1698
Publisher: Elsevier Ltd
DOI: 10.1016/j.tiv.2013.04.015
ISSN: 1879-3177
PMID: 23665401
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health
Language: English
Record Identifier: 99685297702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Toxicology

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