Journal article
Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora
Acta Physiologiae Plantarum, Vol.34(1), pp.117-128
2012
Abstract
A protocol was developed for short-term preservation and distribution of the plantation eucalypt, Corymbia torelliana 9 C. Citriodora, using alginateencapsulated shoot tips and nodes as synthetic seeds. Effects of sowing medium, auxin concentration, storage temperature and planting substrate on shoot regrowth or conversion into plantlets were assessed for four different clones. High frequencies of shoot regrowth (76-100%) from encapsulated explants were consistently obtained in hormone-free half- and full-strength Murashige and Skoog (MS) sowing media. Conversion into plantlets from synthetic seeds was achieved on half-strength MS medium by treating shoot tips or nodes with 4.9-78.4 lM IBA prior to encapsulation. Pre-treatment with 19.6 lM IBA provided 62-100% conversion, and 95-100% of plantlets survived after acclimatisation under nursery conditions. Synthetic seeds containing explants pre-treated with IBA were stored for 8 weeks much more effectively at 25oC than at 4oC, with regrowth frequencies of 50-84% at 25oC compared with 0-4% at 4oC. To eliminate the in vitro culture step after encapsulation, synthetic seeds were allowed to preconvert before sowing directly onto a range of ex vitro nonsterile planting substrates. Highest frequencies (46-90%) of plantlet formation from pre-converted synthetic seeds were obtained by transferring shoot tip-derived synthetic seeds onto an organic compost substrate. These plantlets exhibited almost 100% survival in the nursery without mist irrigation. Pre-conversion of non-embryonic synthetic seeds is a novel technique that provides a convenient alternative to somatic embryo-derived artificial seeds
Details
- Title
- Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora
- Authors
- Cao Dinh Hung (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringStephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Acta Physiologiae Plantarum, Vol.34(1), pp.117-128
- Publisher
- Springer Verlag
- Date published
- 2012
- DOI
- 10.1007/s11738-011-0810-0
- ISSN
- 0137-5881; 0137-5881
- Copyright note
- Copyright © 2012 Springer-Verlag. The Author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final publication is available at www.springerlink.com
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99450002702621
- Output Type
- Journal article
- Research Statement
- false
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