Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora

Cao Dinh Hung; Stephen J Trueman

doi:10.1007/s11738-011-0810-0

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Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora

Journal article

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Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora

Cao Dinh Hung and Stephen J Trueman

Acta Physiologiae Plantarum, Vol.34(1), pp.117-128

2012

DOI: https://doi.org/10.1007/s11738-011-0810-0

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Abstract

advantitious roots

Eucalyptus

plantlet

propagation

synthetic seeds

tissue culture

A protocol was developed for short-term preservation and distribution of the plantation eucalypt, Corymbia torelliana 9 C. Citriodora, using alginateencapsulated shoot tips and nodes as synthetic seeds. Effects of sowing medium, auxin concentration, storage temperature and planting substrate on shoot regrowth or conversion into plantlets were assessed for four different clones. High frequencies of shoot regrowth (76-100%) from encapsulated explants were consistently obtained in hormone-free half- and full-strength Murashige and Skoog (MS) sowing media. Conversion into plantlets from synthetic seeds was achieved on half-strength MS medium by treating shoot tips or nodes with 4.9-78.4 lM IBA prior to encapsulation. Pre-treatment with 19.6 lM IBA provided 62-100% conversion, and 95-100% of plantlets survived after acclimatisation under nursery conditions. Synthetic seeds containing explants pre-treated with IBA were stored for 8 weeks much more effectively at 25oC than at 4oC, with regrowth frequencies of 50-84% at 25oC compared with 0-4% at 4oC. To eliminate the in vitro culture step after encapsulation, synthetic seeds were allowed to preconvert before sowing directly onto a range of ex vitro nonsterile planting substrates. Highest frequencies (46-90%) of plantlet formation from pre-converted synthetic seeds were obtained by transferring shoot tip-derived synthetic seeds onto an organic compost substrate. These plantlets exhibited almost 100% survival in the nursery without mist irrigation. Pre-conversion of non-embryonic synthetic seeds is a novel technique that provides a convenient alternative to somatic embryo-derived artificial seeds

Details

Title: Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora
Authors: Cao Dinh Hung (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Stephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Acta Physiologiae Plantarum, Vol.34(1), pp.117-128
Publisher: Springer Verlag
Date published: 2012
DOI: 10.1007/s11738-011-0810-0
ISSN: 0137-5881
Copyright note: Copyright © 2012 Springer-Verlag. The Author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final publication is available at www.springerlink.com
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99450002702621
Output Type: Journal article

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