Advancing DNA Barcoding and Metabarcoding Applications for Plants Requires Systematic Analysis of Herbarium Collections—An Australian Perspective

Eleanor E Dormontt; Kor-jent Van Dijk; Karen L Bell; Ed Biffin; Martin F Breed; Margaret Byrne; Stefan Caddy-Retalic; Francisco Encinas-Viso; Paul Neville; Alison Shapcott; Jennifer M Young; Michelle Waycott; Andrew J Lowe

doi:10.3389/fevo.2018.00134

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Advancing DNA Barcoding and Metabarcoding Applications for Plants Requires Systematic Analysis of Herbarium Collections—An Australian Perspective

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Advancing DNA Barcoding and Metabarcoding Applications for Plants Requires Systematic Analysis of Herbarium Collections—An Australian Perspective

Eleanor E Dormontt, Kor-jent Van Dijk, Karen L Bell, Ed Biffin, Martin F Breed, Margaret Byrne, Stefan Caddy-Retalic, Francisco Encinas-Viso, Paul Neville, Alison Shapcott, …

Frontiers in Ecology and Evolution, Vol.6, 134

2018

DOI: https://doi.org/10.3389/fevo.2018.00134

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Abstract

natural history collections

barcoding reference database

conservation

science infrastructure

taxonomy

Building DNA barcode databases for plants has historically been ad hoc, and often with a relatively narrow taxonomic focus. To realise the full potential of DNA barcoding for plants, and particularly its application to metabarcoding for mixed-species environmental samples, systematic sequencing of reference collections is required using an augmented set of DNA barcode loci, applied according to agreed data generation and analysis standards. The largest and most complete reference collections of plants are held in herbaria. Australia has a globally significant flora that is well sampled and expertly curated by its herbaria, coordinated through the Council of Heads of Australasian Herbaria. There exists a tremendous opportunity to provide a comprehensive and taxonomically robust reference database for plant DNA barcoding applications by undertaking coordinated and systematic sequencing of the entire flora of Australia utilising existing herbarium material. In this paper, we review the development of DNA barcoding and metabarcoding and consider the requirements for a robust and comprehensive system. We analysed the current availability of DNA barcode reference data for Australian plants, recommend priority taxa for database inclusion and highlight future applications of a comprehensive metabarcoding system. We urge that large-scale and coordinated analysis of herbarium collections be undertaken to realise the promise of DNA barcoding and metabarcoding, and propose that the generation and curation of reference data should become a national investment priority.

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Title: Advancing DNA Barcoding and Metabarcoding Applications for Plants Requires Systematic Analysis of Herbarium Collections—An Australian Perspective
Authors: Eleanor E Dormontt (Author) - University of Adelaide
Kor-jent Van Dijk (Author) - University of Adelaide
Karen L Bell (Author) - University of Western Australia
Ed Biffin (Author) - State Herbarium of South Australia
Martin F Breed (Author) - University of Adelaide
Margaret Byrne (Author) - Conservation and Attractions (DBCA)
Stefan Caddy-Retalic (Author) - University of Adelaide
Francisco Encinas-Viso (Author) - Centre for Australian National Biodiversity Research (CSIRO)
Paul Neville (Author) - Curtin University
Alison Shapcott (Author) - University of Adelaide
Jennifer M Young (Author) - University of Adelaide
Michelle Waycott (Author) - University of Adelaide
Andrew J Lowe (Author) - University of Adelaide
Publication details: Frontiers in Ecology and Evolution, Vol.6, 134; 12
Publisher: Frontiers Research Foundation
Date published: 2018
DOI: 10.3389/fevo.2018.00134
ISSN: 2296-701X
Copyright note: Copyright © 2018 Dormontt, Van Dijk, Bell, Biffin, Breed, Byrne, Caddy-Retalic, Encinas-Viso, Neville, Shapcott, Young, Waycott and Lowe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451124502621
Output Type: Journal article

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