Acinetobacter baumannii detected on modified charcoal-cefoperazone-deoxycholate agar in a waste stabilisation pond

Maxim Sheludchenko; Anna Padovan; Mohammad Katouli; Helen Stratton

doi:10.1139/cjm-2019-0282

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Acinetobacter baumannii detected on modified charcoal-cefoperazone-deoxycholate agar in a waste stabilisation pond

Journal article

Peer reviewed

Acinetobacter baumannii detected on modified charcoal-cefoperazone-deoxycholate agar in a waste stabilisation pond

Maxim Sheludchenko, Anna Padovan, Mohammad Katouli and Helen Stratton

Canadian Journal of Microbiology, Vol.66(3), pp.206-213

2020

DOI: https://doi.org/10.1139/cjm-2019-0282

PMID: 31855468

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Abstract

Acinetobacter baumannii

bacterial survival

mCCDA agar

waste stabilization pond (WSP)

Campylobacter is a recommended reference pathogen for the verification and validation of water recycling schemes in Australia and globally. In a larger study investigating the efficacy of pathogen removal in waste stabilization ponds (WSP), we cultivated bacteria from wastewater samples on modified charcoal–cefoperazone–deoxycholate agar (mCCDA) targeting the growth of Campylobacter. A high number of colonies characteristic of Campylobacter grew on this selective medium, but this did not correlate with qPCR data. Using primers targeting the 16S rRNA gene, and additional confirmatory tests to detect VS1, ompA, bla OXA-51-like , bla OXA-23-like genes, we tested 80 random colonies from 10 WSP samples. All 80 were identified as Acinetobacter baumannii. Wastewater grab samples taken three times over 6 months throughout the WSP system showed removal of A. baumannii in the WSP at rates similar to that of Escherichia coli. Our study suggests that mCCDA agar is not a suitable medium for isolating Campylobacter from environmental samples and that A. baumannii can be used as an indicator for removal of pathogens in WSPs.

Details

Title: Acinetobacter baumannii detected on modified charcoal-cefoperazone-deoxycholate agar in a waste stabilisation pond
Authors: Maxim Sheludchenko (Author) - Wilmar International Ltd, Singapore
Anna Padovan (Author) - Charles Darwin University
Mohammad Katouli (Author) - University of the Sunshine Coast
Helen Stratton (Author) - Griffith University
Publication details: Canadian Journal of Microbiology, Vol.66(3), pp.206-213
Publisher: Canadian Science Publishing
Date published: 2020
DOI: 10.1139/cjm-2019-0282
ISSN: 0008-4166; 1480-3275
PMID: 31855468
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451418202621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology

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