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Published VersionCC BY V4.0, Open Access
Journal article
Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles
PLoS One, Vol.10(3), pp.1-20
2015
PMID: 25799321
Abstract
Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range. Both appear to form composite α particles from smaller β particles. The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal. This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles. It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans. The monomodal distribution of phytoglycogen decreases uniformly in time with hydrolysis, while with glycogen, the large particles degrade significantly more quickly. The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules. The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely. This finding is of importance for diabetes, where α-particle structure is impaired.
Details
- Title
- Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles
- Authors
- Prudence O. Powell - Huazhong University of Science and TechnologyMitchell A Sullivan - The University of QueenslandJoshua J Sheehy - Huazhong University of Science and TechnologyBenjamin L Schulz - The University of QueenslandFrederick J. Warren - The University of QueenslandRobert G Gilbert (Corresponding Author) - The University of Queensland
- Publication details
- PLoS One, Vol.10(3), pp.1-20
- Publisher
- Public Library of Science
- Date published
- 2015
- DOI
- 10.1371/journal.pone.0121337
- ISSN
- 1932-6203
- PMID
- 25799321
- Copyright note
- © 2015 Powell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- Data Availability
- All relevant data are within the paper and its Supporting Information files.
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035097902621
- Output Type
- Journal article
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- Web Of Science research areas
- Biochemistry & Molecular Biology
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