Journal article
Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR
PLoS One, Vol.8(8), e71647
2013
Abstract
Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei. © 2013 Price et al.
Details
- Title
- Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR
- Authors
- Erin P Price (Author) - Menzies School of Health ResearchDerek S Sarovich (Author) - Menzies School of Health ResearchJ R Webb (Author) - Menzies School of Health ResearchJ L Ginther (Author) - Northern Arizona University, United StatesM Mayo (Author) - Menzies School of Health ResearchJ M Cook (Author) - Northern Arizona University, United StatesM L Seymour (Author) - Northern Arizona University, United StatesM Kaestli (Author) - Menzies School of Health ResearchV Theobald (Author) - Menzies School of Health ResearchC M Hall (Author) - Northern Arizona University, United StatesJ D Busch (Author) - Northern Arizona University, United StatesJ T Foster (Author) - Northern Arizona University, United StatesP Keim (Author) - Northern Arizona University, United StatesD M Wagner (Author) - Northern Arizona University, United StatesA Tuanyok (Author) - Northern Arizona University, United StatesT Pearson (Author) - Northern Arizona University, United StatesB J Currie (Author) - Menzies School of Health Research
- Publication details
- PLoS One, Vol.8(8), e71647
- Publisher
- Public Library of Science
- Date published
- 2013
- DOI
- 10.1371/journal.pone.0071647
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2013 Price et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450522402621
- Output Type
- Journal article
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