A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection

G H Lopez; Melinda M Dean; K Yasui; R M Schuller; F Hirayama; Yoke Lin Fung

doi:10.1111/j.1423-0410.2011.01532.x

Back

A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection

Journal article

Peer reviewed

A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection

G H Lopez, Melinda M Dean, K Yasui, R M Schuller, F Hirayama and Yoke Lin Fung

Vox Sanguinis, Vol.102(2), pp.171-174

2012

DOI: https://doi.org/10.1111/j.1423-0410.2011.01532.x

Files and links (1)

url

https://doi.org/10.1111/j.1423-0410.2011.01532.xView

Published Version

Abstract

cell lines

immunofluorescence

neutrophil antibody screening

There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity.

Details

Title: A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection
Authors: G H Lopez (Author) - Australian Red Cross Blood Service
Melinda M Dean (Author) - Australian Red Cross Blood Service
K Yasui (Author) - Japanese Red Cross, Japan
R M Schuller (Author) - American Red Cross, United States
F Hirayama (Author) - Japanese Red Cross, Japan
Yoke Lin Fung (Author) - University of Queensland
Publication details: Vox Sanguinis, Vol.102(2), pp.171-174
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2012
DOI: 10.1111/j.1423-0410.2011.01532.x
ISSN: 0042-9007
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99448991802621
Output Type: Journal article

Metrics

2 File views/ downloads

388 Record Views

4 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Hematology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites