A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)

Andrea L Taylor; David Mills; Tianfang Wang; Nikoleta Ntalamagka; Scott F Cummins; Abigail Elizur

doi:10.1007/s10126-018-9824-6

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A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)

Journal article

Peer reviewed

A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)

Andrea L Taylor, David Mills, Tianfang Wang, Nikoleta Ntalamagka, Scott F Cummins and Abigail Elizur

Marine Biotechnology, Vol.20(4), pp.531-541

2018

DOI: https://doi.org/10.1007/s10126-018-9824-6

PMID: 29705863

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Abstract

pearl oyster

P. maxima

pheromone

spawning

sperm

protein

Pheromones are considered to play an important role in broadcast spawning in aquatic animals, facilitating synchronous release of gametes. In oysters, the sperm has been implicated as a carrier for the spawn-inducing pheromone (SIP). In hatchery conditions, male pearl oysters (Pinctata maxima) can be stimulated to spawn through a variety of approaches (e.g. rapid temperature change), while females can only be induced to spawn through exposure to conspecific sperm, thus limiting development of targeted pairing, required for genetic research and management. The capacity for commercial production and improvement of genetic lines of pearl oysters could be greatly improved with access to a SIP. In this study, we prepared and sequenced crude and semi-purified P. maxima sperm extracts that were used in bioassays to localise the female SIP. We report that the P. maxima SIP is proteinaceous and extrinsically associated with the sperm membrane. Bioactivity from pooled RP-HPLC fractions, but not individual fractions, suggests that the SIP is multi-component. We conclude that crude sperm preparations, as described in this study, can be used as a sperm-free inducer of female P. maximaspawning, which enables for a more efficient approach to genetic breeding.

Details

Title: A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)
Authors: Andrea L Taylor (Author)
David Mills (Author) - Darwin Aquaculture Centre
Tianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Nikoleta Ntalamagka (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Corresponding Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Marine Biotechnology, Vol.20(4), pp.531-541
Publisher: Springer New York LLC
Date published: 2018
DOI: 10.1007/s10126-018-9824-6
ISSN: 1436-2228
PMID: 29705863
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation; External
Language: English
Record Identifier: 99451460602621
Output Type: Journal article

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