Journal article
A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)
Marine Biotechnology, Vol.20(4), pp.531-541
2018
Abstract
Pheromones are considered to play an important role in broadcast spawning in aquatic animals, facilitating synchronous release of gametes. In oysters, the sperm has been implicated as a carrier for the spawn-inducing pheromone (SIP). In hatchery conditions, male pearl oysters (Pinctata maxima) can be stimulated to spawn through a variety of approaches (e.g. rapid temperature change), while females can only be induced to spawn through exposure to conspecific sperm, thus limiting development of targeted pairing, required for genetic research and management. The capacity for commercial production and improvement of genetic lines of pearl oysters could be greatly improved with access to a SIP. In this study, we prepared and sequenced crude and semi-purified P. maxima sperm extracts that were used in bioassays to localise the female SIP. We report that the P. maxima SIP is proteinaceous and extrinsically associated with the sperm membrane. Bioactivity from pooled RP-HPLC fractions, but not individual fractions, suggests that the SIP is multi-component. We conclude that crude sperm preparations, as described in this study, can be used as a sperm-free inducer of female P. maximaspawning, which enables for a more efficient approach to genetic breeding.
Details
- Title
- A sperm spawn-inducing pheromone in the Silver lip pearl oyster (Pinctada maxima)
- Authors
- Andrea L Taylor (Author)David Mills (Author) - Darwin Aquaculture CentreTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringNikoleta Ntalamagka (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Corresponding Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Marine Biotechnology, Vol.20(4), pp.531-541
- Publisher
- Springer New York LLC
- Date published
- 2018
- DOI
- 10.1007/s10126-018-9824-6
- ISSN
- 1436-2228; 1436-2228
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation; External
- Language
- English
- Record Identifier
- 99451460602621
- Output Type
- Journal article
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- Biotechnology & Applied Microbiology
- Marine & Freshwater Biology
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