A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata: Agamidae)

T Ezaz; Denis O'Meally; A E Quinn; S D Sarre; A Georges; J A Marshall Graves

doi:10.1007/s10616-009-9182-3

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A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata: Agamidae)

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A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata: Agamidae)

T Ezaz, Denis O'Meally, A E Quinn, S D Sarre, A Georges and J A Marshall Graves

Cytotechnology, Vol.58(3), pp.135-139

2008

DOI: https://doi.org/10.1007/s10616-009-9182-3

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Abstract

reptiles

lizards

cell lines

tail

toe

non-invasive chromosomes

Primary cell lines were established from cultures of tail and toe clips of five species of Australian dragon lizards: Tympanocryptis pinguicolla, Tympanocryptis sp., Ctenophorus fordi, Amphibolurus norrisi and Pogona vitticeps. The start of exponential cell growth ranged from 1 to 5 weeks. Cultures from all specimens had fibroblastic morphology. Cell lines were propagated continuously up to ten passages, cryopreserved and recovered successfully. We found no reduction in cell viability after short term (<6 months) storage at -80 °C. Mitotic metaphase chromosomes were harvested from these cell lines and used in differential staining, banding and fluorescent in situ hybridisation. Cell lines maintained normal diploidy in all species. This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice. The method is likely to be applicable to a range of species. Such cell lines provide a virtually unlimited source of material for cytogenetic, evolutionary and genomic studies. © 2009 Springer Science+Business Media B.V.

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Title: A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata: Agamidae)
Authors: T Ezaz (Author) - Australian National University
Denis O'Meally (Author) - Australian National University
A E Quinn (Author) - University of Canberra
S D Sarre (Author) - University of Canberra
A Georges (Author) - University of Canberra
J A Marshall Graves (Author) - Australian National University
Publication details: Cytotechnology, Vol.58(3), pp.135-139
Publisher: Springer Netherlands
Date published: 2008
DOI: 10.1007/s10616-009-9182-3
ISSN: 0920-9069
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449796002621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Cell Biology

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