Journal article
A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria
Letters in Applied Microbiology, Vol.30(3), pp.178-182
2000
Abstract
A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.
Details
- Title
- A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria
- Authors
- J D McKerrow (Author) - La Trobe UniversityS Vagg (Author) - La Trobe UniversityT McKinney (Author) - La Trobe UniversityE M Seviour (Author) - La Trobe UniversityA M Maszennan (Author) - La Trobe UniversityPeter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationR J Seviour (Author) - La Trobe University
- Publication details
- Letters in Applied Microbiology, Vol.30(3), pp.178-182
- Publisher
- Wiley-Blackwell Publishing Ltd.
- DOI
- 10.1046/j.1472-765x.2000.00675.x
- ISSN
- 0266-8254
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation; University of the Sunshine Coast, Queensland; School of Science and Engineering - Legacy
- Language
- English
- Record Identifier
- 99449820502621
- Output Type
- Journal article
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- Biotechnology & Applied Microbiology
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