Journal article
A rapid extraction method for glycogen from formalin-fixed liver
Carbohydrate Polymers, Vol.118, pp.9-15
2015
PMID: 25542100
Abstract
Liver glycogen, a highly branched polymer, acts as our blood-glucose buffer. While past structural studies have extracted glycogen from fresh or frozen tissue using a cold-water, sucrose-gradient centrifugation technique, a method for the extraction of glycogen from formalin-fixed liver would allow the analysis of glycogen from human tissues that are routinely collected in pathology laboratories. In this study, both sucrose-gradient and formalin-fixed extraction techniques were carried out on piglet livers, with the yields, purities and size distributions (using size exclusion chromatography) compared. The formalin extraction technique, when combined with a protease treatment, resulted in higher yields (but lower purities) of glycogen with size distributions similar to the sucrose-gradient centrifugation technique. This formalin extraction procedure was also significantly faster, allowing glycogen extraction throughput to increase by an order of magnitude. Both extraction techniques were compatible with mass spectrometry proteomics, with analysis showing the two techniques were highly complementary.
Details
- Title
- A rapid extraction method for glycogen from formalin-fixed liver
- Authors
- Mitchell A Sullivan - University of QueenslandShihan Li - Huazhong University of Science and TechnologySamuel T. N. Aroney - University of QueenslandBin Deng - Huazhong University of Science and TechnologyCheng Li - University of QueenslandEugeni Roura - University of QueenslandBenjamin L Schulz - University of QueenslandBrooke E Harcourt - Translational Research InstituteJosephine M Forbes - Translational Research InstituteRobert G. Gilbert (Corresponding Author) - Huazhong University of Science and Technology
- Publication details
- Carbohydrate Polymers, Vol.118, pp.9-15
- Publisher
- Elsevier Ltd
- Date published
- 2015
- DOI
- 10.1016/j.carbpol.2014.11.005
- ISSN
- 1879-1344
- PMID
- 25542100
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035098002621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Applied
- Chemistry, Organic
- Polymer Science
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