A pyrazolopyrimidine based fluorescent probe for the detection of Cu2+ and Ni2+ and its application in living cells

Yun-Qiong Gu; Wen-Ying Shen; Yan Zhou; Shan-Feng Chen; Yan Mi; Bing-Fan Long; David James Young; Fei-Long Hu

doi:10.1016/j.saa.2018.10.030

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A pyrazolopyrimidine based fluorescent probe for the detection of Cu2+ and Ni2+ and its application in living cells

Journal article

Open access

Peer reviewed

A pyrazolopyrimidine based fluorescent probe for the detection of Cu2+ and Ni2+ and its application in living cells

Yun-Qiong Gu, Wen-Ying Shen, Yan Zhou, Shan-Feng Chen, Yan Mi, Bing-Fan Long, David James Young and Fei-Long Hu

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol.209, pp.141-149

2018

DOI: https://doi.org/10.1016/j.saa.2018.10.030

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Abstract

Cu2+ sensing

Ni2+ sensing

fluorescent sensor

pyrazolopyrimidines

A fluorescent sensor L based on a pyrazolopyrimidine core simultaneously detects Cu2+ and Ni2+ ions by photoluminescence quenching, even in the presence of other metal cations. Sensor L possesses high association constants of 5.24 × 103 M-1 and 2.85 × 104 M-1 and low detection limits of 0.043 μM and 0.038 μM for Cu2+ and Ni2+, respectively. The binding stoichiometry ratios of L to Cu2+ or Ni2+ is 1:1 as determined by Benesi-Hildebrand and Job's plots, and by crystal structures. DFT calculations on L-Cu2+ indicated reduced electron donation from the coordinated pyrazolopyridine to the fused pyrimidine and pendant phenyl group which, together with a smaller HOMO-LUMO orbital gap could favour non-radiative decay and explain the observed fluorescence quenching. Sensor L possessed low cytotoxicity and good imaging characteristics for Cu2+ and Ni2+ in living cells, suggesting potential applications for detecting Cu2+ and Ni2+in vivo.

Details

Title: A pyrazolopyrimidine based fluorescent probe for the detection of Cu2+ and Ni2+ and its application in living cells
Authors: Yun-Qiong Gu (Author) - Guangxi Teachers Education University, China
Wen-Ying Shen (Author) - Guangxi Normal University, China
Yan Zhou (Author) - Guangxi University for Nationalities, China
Shan-Feng Chen (Author) - Guangxi University for Nationalities, China
Yan Mi (Author) - Guangxi University for Nationalities, China
Bing-Fan Long (Author) - Guangxi University for Nationalities, China
David James Young (Author) - Guangxi Teachers Education University, China
Fei-Long Hu (Author) - Guangxi University for Nationalities, China
Publication details: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol.209, pp.141-149
Publisher: Elsevier BV
Date published: 2018
DOI: 10.1016/j.saa.2018.10.030
ISSN: 1386-1425; 1386-1425
Copyright note: Copyright © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451458202621
Output Type: Journal article

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Web Of Science research areas: Spectroscopy

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