A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis

Samantha J Nowland; Dean R Jerry; Paul C Southgate

doi:10.1111/ivb.12091

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A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis

Journal article

Peer reviewed

A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis

Samantha J Nowland, Dean R Jerry and Paul C Southgate

Invertebrate Biology, Vol.134(3), pp.252-259

2015

DOI: https://doi.org/10.1111/ivb.12091

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Abstract

sea cucumber

sandfish

Holothuria scabra

endangered species

Increasing demand and overfishing of high-value species has promoted interest in both conservation and aquaculture initiatives supporting stock restoration programs for tropical sea cucumbers. Accordingly, there is a need for baseline information on the genetic structures and relatedness of sea cucumber populations to support sustainable implementation of mariculture and conservation programs, which often involve coastal communities in developing countries. Identification of a non-destructive tissue sampling technique for sea cucumbers that allows extraction of high-quality genomic DNA in a sustainable and culturally appropriate way is thus required. Six sampling techniques were assessed for their suitability to collect tissue for DNA extraction from sandfish (Holothuria scabra): core needle biopsy, punch biopsy, shave biopsy, buccal swab, anal swab, and evisceration. The quantity, quality, and purity of extracted DNA were compared to assess the relative merit of each sampling method. The swab biopsy method produced the best quality DNA agarose band image, and evisceration resulted in the highest yields of DNA, at an average of 525.9 (±98.0) μg g-1. However, when considering all criteria assessed, the swab biopsy methods (both buccal and anal) proved superior. Swabbing not only produced the best quality DNA agarose band image, it was also the only technique that produced DNA that amplified 100% of the time across both extraction protocols. We hope that the non-destructive sampling techniques evaluated in this study provide a foundation for the genetic analysis of sea cucumber stocks to support their conservation and management.

Details

Title: A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis
Authors: Samantha J Nowland (Author) - James Cook University
Dean R Jerry (Author) - James Cook University
Paul C Southgate (Author) - James Cook University
Publication details: Invertebrate Biology, Vol.134(3), pp.252-259
Publisher: Wiley-Blackwell Publishing Inc.
Date published: 2015
DOI: 10.1111/ivb.12091
ISSN: 1077-8306
Organisation Unit: School of Science and Engineering - Legacy; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449382102621
Output Type: Journal article

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